Abstract
Strain WRN001T, a Gram-staining-negative, strictly aerobic, non-motile bacterium was isolated from the natural saline-alkali wetland soil of Binhai new district, Tianjin, China (38°46’ N, 117°13’ E). Cells of strain WRN001T were 0.3–0.5 µm in width and 1.5–2.5 µm in length, and the growth occurred optimally at 33–37 °C, pH 7.5–8.0, and in the presence of 8–10% (w/v) NaCl. Based on 16S rRNA gene sequence analysis, the isolate could be affiliated to the genus Halomonas, and the highest 16S rRNA gene sequence similarity of strain WRN001T to its closest relative Halomonas qiaohouensis DSM 26770 T was 97.5%. The size of the genome as presented here was 5,475,884 bp with a G + C content of 63.8 mol %. The major respiratory quinone of strainWRN001T was Q-9, and the dominant fatty acids were summed feature 8, summed feature 3, C10:0, C12:0, C12:0 3-OH, C16:0, and C17:0 cyclo. The major polar lipids were diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE), phosphatidylglycerol (PG), phophatidylcholine (PC), two phospholipids (PL), aminolipid (AL), and three unidentified lipids (L). These data combined with the low digital DDH values between strain WRN001T and the close relative, Halomonas alkalitolerans CGMCC 1.9129 T (42.2%) and based on comparisons with currently available genomes, the highest average nucleotide identity (ANIm) value was 91.4% to Halomonas alkalitolerans CGMCC 1.9129 T (GenBank accession No. GCA_001971685.1). Therefore, we propose a novel species in the genus Halomonas to accommodate this novel isolate: Halomonas salipaludis sp. nov. (type strain WRN001T = KCTC 52853 T = ACCC 19974 T).
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Data availability
The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene of Halomonas salipaludis sp. nov. strain WRN001T is MF782428. The whole genome was deposited at GenBank/EMBL/DDBJ under the accession number NSKB00000000 for strain WRN001T. Transmission electron micrographs (TEM) of cells of strain WRN001T, thin-layer chromatograms of the polar lipids extracted from strain WRN001T and closely related species, additional phylogenetic trees, and the table containing the average nucleotide identity (ANI), and digital DDH values to closely related genomes are available as Supplementary Materials.
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Acknowledgements
This work was supported by the National Natural Science Foundation of China (NSFC No. 31670113). We would like to thank Prof. Aharon Oren for very valuable help in naming the organism.
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This work was supported by the National Natural Science Foundation of China (NSFC No. 31670113).
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JX contributed to performing the experiments and writing the initial draft. QG provided samples of experiment and participated in the isolation and cultivation of strains. GZ and JZ contributed to the guidance of experimental operations. LT and JL contributed to the morphological analyzes. HF, XW and HL performed genome analysis. GZ and JZ contributed to reagents, instrumentation, and the financial support for this work.
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Xing, J., Gong, Q., Tang, L. et al. Halomonas salipaludis sp. nov., isolated from a saline-alkali wetland soil. Arch Microbiol 203, 6033–6039 (2021). https://doi.org/10.1007/s00203-021-02560-8
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DOI: https://doi.org/10.1007/s00203-021-02560-8