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Bradyrhizobium japonicum, B. elkanii and B. diazoefficiens Interact with Rice (Oryza sativa), Promote Growth and Increase Yield

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Abstract

Bradyrhizobium is a genus of plant growth-promoting rhizobacteria (PGPR) that have been studied for several decades mainly for the ability to fix diazotrophic nitrogen after having been established endosymbiotically inside root nodules of the legumes of Fabaceae. The aim of this work was to evaluate the capability of Bradyrhizobium to promote the growth of crops belonging to other families, in this case, rice (Oryza sativa), both in laboratory and in field trials. For laboratory test, surface-sterilized rice seeds were soaked with cultures of each strain and planted in pots. Plant length and dry weight were measured after 35 days. For the field test, rice seeds of varieties Yeruá La Plata and Gurí INTA were inoculated with the three best strains observed in the laboratory test and planted in plots. After 60 days of growth, plant length and dry weight were measured. At harvest time, we measured the dry weight of the aerial part, yield and thousand-grain weight. Inoculation with any of the three species described provoked significant increments compared to the uninoculated control at least in one of the parameters measured, both in the laboratory and in the field tests. Bradyrhizobium japonicum E109 was the strain that promoted rice growth the most in the lab while Bradyrhizobium elkanii SEMIA 587 was the strain that promoted rice growth the most in the field, with increments in yield of approximately 1000 kg/ha. Data obtained suggest that the Bradyrhizobium species promoted all rice growth and yield.

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Acknowledgements

D.P. was supported by a grant from National Science Foundation, Colombo, Sri Lanka. J.M.R. was supported by a grant from the International Centre for Genetic Engineering and Biotechnology (ICGEB), Buenos Aires, Argentina. P.A.B. is a scientist supported by the Comisión de Investigaciones Científicas de la Provincia de Buenos Aires. This research was also possible thanks to funds made available by ICGEB and the support from the Faculty of Agriculture, University of Ruhuna, Mapalana, Kamburupitiya, Sri Lanka, and Facultad de Ciencias Agrarias y Forestales, Universidad Nacional de La Plata (FCAyF).

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Authors and Affiliations

  1. National Science Foundation, Maitland Place, Colombo, Sri Lanka

    Duwini Padukkage

  2. Faculty of Agriculture, University of Ruhuna, Mapalana, Kamburupitiya, Sri Lanka

    Sudarshanee Geekiyanage

  3. Centro de Investigaciones de Fitopatologia (CIDEFI), Facultad de Ciencias Agrarias Y Forestales, Universidad Nacional de La Plata (FCAyF), Comisión de Investigaciones Científicas de La Provincia de Buenos Aires, Buenos Aires, Argentina

    Juan M. Reparaz, Rodolfo Bezus & Pedro A. Balatti

  4. International Centre for Genetic Engineering and Biotechnology, ICGEB, Buenos Aires, Argentina

    Giuliano Degrassi

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  1. Duwini Padukkage
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DP performed the experiments in the laboratory; JMR and RB followed the field trials; SG, PAB and GD planned the experiments, elaborated the results and wrote the manuscript.

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Correspondence to Giuliano Degrassi.

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Padukkage, D., Geekiyanage, S., Reparaz, J.M. et al. Bradyrhizobium japonicum, B. elkanii and B. diazoefficiens Interact with Rice (Oryza sativa), Promote Growth and Increase Yield. Curr Microbiol 78, 417–428 (2021). https://doi.org/10.1007/s00284-020-02249-z

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