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Effects of NADH Availability on 3-Phenyllactic Acid Production by Lactobacillus plantarum Expressing Formate Dehydrogenase

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Abstract

It is well known that cofactors play a key role in the production of different compounds in bioconversion processes, while the high cost of cofactors limits their usage in industrial applications. In the present study, a NADH regeneration system was successfully developed in Lactobacillus plantarum by expressing the fdh gene coding for formate dehydrogenase (FDH) from Candida boidinii. Results indicated that the FDH was expressed with the highest activity of 0.82 U/mg of protein when cells entered early stationary phase. In addition, the expression of FDH increased the intracellular level of NADH and NADH/NAD+ ratio in L. plantarum, and therefore, enhanced the NADH-dependent production of 3-phenyllactic acid (PLA) in repeated and fed-batch bioconversions. In brief, the results demonstrate that the NADH regeneration by expressing FDH is a promising strategy for producing NADH-dependent microbial metabolites in L. plantarum.

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Acknowledgements

This research was supported by Natural Science Foundation of the Higher Education Institutions of Jiangsu Province (Grant no. 16KJB180033).

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Authors and Affiliations

  1. School of Pharmaceutical Engineering, Jiangsu Food and Pharmaceutical Science College, Huai’an, 223003, Jiangsu, China

    Minghua Li, Chunjie Zhu & Huiying Ji

  2. Jiangsu Engineering Laboratory of Protein Drugs, Huai’an, 223003, Jiangsu, China

    Minghua Li & Chunjie Zhu

  3. School of Food, Jiangsu Food and Pharmaceutical Science College, Huai’an, 223003, Jiangsu, China

    Xiumei Meng & Zhiyang Sun

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  1. Minghua Li
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Correspondence to Minghua Li.

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Li, M., Meng, X., Sun, Z. et al. Effects of NADH Availability on 3-Phenyllactic Acid Production by Lactobacillus plantarum Expressing Formate Dehydrogenase. Curr Microbiol 76, 706–712 (2019). https://doi.org/10.1007/s00284-019-01681-0

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  • DOI: https://doi.org/10.1007/s00284-019-01681-0

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