Abstract
The healing properties of hyaluronic acid (HA) in the recovery of wounds are well known. Cefoxitin (Cef), a cephalosporin antibiotic, is generally used to prevent and treat postoperative infections. In this study, we describe the incorporation of Cef in HA thin films (Cef–HAF) by using electrospraying. Scanning electron microscopy images showed that HA-containing thin films (HAF) were composed of numerous nanoparticles (255 ± 177 nm in diameter) with irregular surfaces, connected to each other with nanofibers of 50 ± 11 nm in diameter. Cef–HAF contained fewer, but larger, particles (551 ± 293 nm) with smooth surfaces and were interconnected with nanofibers of 61 ± 13 nm in diameter. Differences in surface morphology between HAF and Cef–HAF were confirmed by atomic force microscopy. Fourier transform infrared and X-ray diffraction analyses revealed that Cef was not modified when incorporated into Cef–HAF and remained active against Klebsiella pneumoniae Xen 39, Staphylococcus aureus Xen 36 and Listeria monocytogenes EDGe. Nanofiber scaffolds of HA-containing Cef may be used in dressings to control postoperative infections.
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Acknowledgments
We are thankful to Mrs. Madelaine Frazenburg, Electron Microbeam Unit, Central Analytical Facilities, Stellenbosch University for her assistance with FEG–SEM analyses. Ahire J. J. is grateful to Stellenbosch University for a Postdoctoral Fellowship.
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284_2016_1057_MOESM1_ESM.tif
(a) Collector for the deposition of thin films by electrospraying. Digital images of thin films after drying, (b) hyaluronic acid thin film (HAF) without transparent plastic support, (c) Cefoxitin-hyaluronic acid thin film (Cef-HAF) with transparent plastic support (TIFF 4344 kb)
284_2016_1057_MOESM2_ESM.tif
X-ray diffraction (XRD) pattern of Cefoxitin (Cef), hyaluronic acid thin film (HAF), and cefoxitin-hyaluronic acid thin film (Cef-HAF). Arrow: Peaks corresponding to Cef in Cef-HAF (TIFF 3730 kb)
284_2016_1057_MOESM3_ESM.tif
In vitro antimicrobial activity of hyaluronic acid thin film (HAF), and cefoxitin-hyaluronic acid thin film (Cef-HAF) against S. aureus Xen 36, L. monocytogenes EDGe, K. pneumonia Xen 39 and P. aeruginosa PA01 (TIFF 490 kb)
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Ahire, J.J., Dicks, L.M.T. Antimicrobial Hyaluronic Acid–Cefoxitin Sodium Thin Films Produced by Electrospraying. Curr Microbiol 73, 236–241 (2016). https://doi.org/10.1007/s00284-016-1057-1
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DOI: https://doi.org/10.1007/s00284-016-1057-1