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Sphingomonas qilianensis sp. nov., Isolated from Surface Soil in the Permafrost Region of Qilian Mountains, China

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Abstract

A Gram-stain-negative, strictly aerobic, non-motile and rod-shaped bacterial strain, designated X1T, was isolated from the permafrost region of Qilian Mountains in northwest of China. Phylogenetic analyses of 16S rRNA gene sequence revealed that strain X1T was a member of the genus Sphingomonas and shared the highest 16S rRNA gene sequence similarity with Sphingomonas oligophenolica JCM 12082T (96.9 %), followed by Sphingomonas glacialis CGMCC 1.8957T (96.7 %) and Sphingomonas alpina DSM 22537T (96.4 %). Strain X1T was able to grow at 15–30 °C, pH 6.0–10.0 and with 0–0.3 % NaCl (w/v). The DNA G+C content of the isolate was 64.8 mol%. Strain X1T-contained Q-10 as the dominant ubiquinone and C18:1 ω7c, C16:1 ω7c, C16:0 and C14:0 2-OH as the dominant fatty acids. The polar lipid profile of strain XIT-contained sphingoglycolipid, phosphatidylglycerol, phosphatidylethanolamine, one unidentified glycolipid and two unidentified phospholipid. Due to the phenotypic and genetic distinctiveness and other characteristic studied in this article, we consider X1T as a novel species of the genus Sphingomonas and propose to name it Sphingomonas qilianensis sp. nov. The type strain is X1T (=CGMCC 1.15349T = KCTC 42862T).

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Authors and Affiliations

  1. College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, 100029, People’s Republic of China

    Ai-Lian Piao, Xiao-Min Feng, Lu Han & Jie Lv

  2. Research and Development Center for Marine Biosciences, Japan Agency for Marine-Earth Science and Technology (JAMSTEC), 2-15 Natsushima-cho, Yokosuka, 237-0061, Japan

    Yuichi Nogi

  3. Qinghai No.105 Coal Geological Exploration Team, Xining, 810007, Qinghai, China

    Yonghong Li

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  1. Ai-Lian Piao
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  2. Xiao-Min Feng
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  3. Yuichi Nogi
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  4. Lu Han
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Corresponding author

Correspondence to Jie Lv.

Additional information

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain X1T is KT000387.

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Piao, AL., Feng, XM., Nogi, Y. et al. Sphingomonas qilianensis sp. nov., Isolated from Surface Soil in the Permafrost Region of Qilian Mountains, China. Curr Microbiol 72, 363–369 (2016). https://doi.org/10.1007/s00284-015-0957-9

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  • DOI: https://doi.org/10.1007/s00284-015-0957-9

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