Abstract
GABA accumulation and glutamate decarboxylase (GAD) activity, the principal enzyme involved in GABA formation, was investigated in cyanobacterium Synechocystis sp. PCC 6803 wild-type (WT) and gad knockout mutant strains grown in medium containing different nitrogenous compounds. Nitrate was the best nitrogen source for GAD activity and GABA accumulation followed by nitrite, ammonium, and urea. An increase in the accumulation of GABA was observed in WT and mutant cells grown for 24 h in medium supplemented with 0.5 mM putrescine or spermidine with a parallel increase in GAD activity. The mutant could not accumulate GABA at all the conditions tested except when supplemented with putrescine or spermidine, where high GABA levels were observed in both WT and mutant strains. Glutamate supplementation up to 10 mM for 24 h resulted in a significant increase in both GAD activity and GABA content. Overall results suggested that optimization of nitrogen source and nitrogenous compounds supplementation was effective for the enhancement of GABA accumulation in Synechocystis.
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Acknowledgments
Simab Kanwal thanks Department of Biochemistry, Faculty of Science and the 90th Anniversary of Chulalongkorn University Ratchadaphiseksomphot Endowment Fund for a PhD scholarship. Wanthanee Khetkorn thanks the Graduate School of Chulalongkorn University for providing postdoctoral fellowship. Aran Incharoensakdi thanks the Ratchadaphiseksomphot Endowment Fund of Chulalongkorn University and the Office of Higher Education Commission for the research grants RES560530052-FW and WCU-013-FW-57, respectively.
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Kanwal, S., Khetkorn, W. & Incharoensakdi, A. GABA Accumulation in Response to Different Nitrogenous Compounds in Unicellular Cyanobacterium Synechocystis sp. PCC 6803. Curr Microbiol 70, 96–102 (2015). https://doi.org/10.1007/s00284-014-0687-4
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DOI: https://doi.org/10.1007/s00284-014-0687-4