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Clinicopathological features of HCV-positive splenic diffuse large B cell lymphoma

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Abstract

The hepatitis C virus (HCV) is a single-stranded RNA virus which is thought to be involved in the onset of B cell lymphoma. HCV-positive diffuse large B cell lymphoma (DLBCL) has been reported to clinically manifest in extranodal lesions (e.g., in the liver, spleen, and stomach). Here, we investigated HCV-positive and -negative primary splenic DLBCL (p-spDLBCL) and non-primary splenic DLBCL (ordinary DLBCL). Furthermore, to examine HCV lymphomagenesis, RNA in situ hybridization (ISH), RT-PCR (reverse-transcription polymerase chain reaction), and NS3 immunostaining of HCV viral nonstructural proteins were performed. HCV-positive p-spDLBCL patients presented fewer B symptoms (asymptomatic) and better performance status, with elevated presence of splenic macronodular lesions and more germinal center B cell (GCB) sub-group cases than HCV-negative p-spDLBCL patients. However, HCV-positive ordinary DLBCL patients were found to have more non-GCB sub-group cases than HCV-negative ordinary DLBCL patients. HCV-positive DLBCL patients showed 20.6% (7/34) NS3 positivity, 16.7% (1/6) HCV-RNA in situ positivity, and 22.2% (2/9) detection of HCV-RNA in tumor tissue by RT-PCR. Splenic samples were found to have a higher frequency of HCV detection than lymph node samples, thus suggesting that HCV may be closely related to lymphomagenesis, especially in splenic lymphoma.

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Acknowledgments

The authors thank Kazutaka Nakashima, Mayumi Miura, Kanoko Miyazaki, Yuki Morotomi, Chie Kuroki, and Kaoruko Nagatomo for their technical assistance.

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Authors and Affiliations

  1. Department of Pathology, Kurume University, School of Medicine, Asahimachi 67, Kurume, Fukuoka, 830-0011, Japan

    Joji Shimono, Hiroaki Miyoshi, Fumiko Arakawa, Kyohei Yamada & Koichi Ohshima

  2. Department of Hematology, Hokkaido University Faculty of Medicine, Sapporo, Japan

    Joji Shimono & Takanori Teshima

  3. Department of Medicine and Biosystemic Science, Kyushu University Graduate School of Medical Sciences, Fukuoka, Japan

    Takeshi Sugio, Kohta Miyawaki, Koji Kato & Koichi Akashi

  4. Department of Hematology, Hamanomachi Hospital, Fukuoka, Japan

    Tetsuya Eto

  5. Department of Hematology, Kushiro Rosai Hospital, Kushiro, Japan

    Takuto Miyagishima

  6. Department of Hematology, Kurume University, School of Medicine, Kurume, Japan

    Koji Nagafuji

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  1. Joji Shimono
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Contributions

J.S. and H.M. were responsible for conception and design; T.S., K.M., T.E., T.M., K.K., K.N., and K.A. provided study materials or patients; J.S., F.A., K.Y., T.S., K.M., T.E., T.M., K.K., K.N., and K.A. performed the collection and assembly of data; J.S., H.M., T.T., and K.O. performed data analysis and interpretation; J.S. wrote the manuscript; and all authors approved the final manuscript.

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Correspondence to Hiroaki Miyoshi.

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The use of clinical samples and information was approved by the Research Ethics Committee of Kurume University and was performed in accordance with the Declaration of Helsinki.

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Shimono, J., Miyoshi, H., Arakawa, F. et al. Clinicopathological features of HCV-positive splenic diffuse large B cell lymphoma. Ann Hematol 98, 1197–1207 (2019). https://doi.org/10.1007/s00277-019-03628-8

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