Abstract
Phloroglucinol is an important chemical intermediate which has been tentatively produced by engineered bacteria. However, its biosynthesis in industry is limited due to its natural antibacterial activity. Our study firstly selected Yarrowia lipolytica as the chassis strain, which was verified to be tolerable to phloroglucinol. Then the gene of type III polyketone synthase PhlD (the key biosynthetic gene) was overexpressed to facilitate phloroglucinol production with a concentration of 107.4 mg/L. Furthermore, we introduced the prokaryotic nanocompartment to assist the intracellular catalytic activity. The results showed that the concentration of phloroglucinol was increased by about 2.5 times, indicating this multifunctional nanocompartment is orthogonal to the physiological activities of Y. lipolytica. Additionally, fermentations with xylose and lignocellulosic hydrolysates as the carbon source were performed with the engineered Y. lipolytica, resulting in a total concentration of 580.2 mg/L and 328.9 mg/L, respectively. These findings revealed the potential of Y. lipolytica in phloroglucinol production and provided an effective nanocompartment strategy to improve the catalytic activity of the enzyme for boosting phloroglucinol production.
Key points
• The first time to select and use Y. lipolytica to produce phloroglucinol.
• Successful construction of prokaryotic nanocompartment in Y. lipolytica to increase production of phloroglucinol.
• Lignocellulose hydrolysate is used as a substrate in fermentation.
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All data generated or analyzed during this study are included in this published article (and its supplementary information files).
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Acknowledgements
The authors thank the public platform of Laboratory of Industrial Catalysis& Metabolic Engineering (Nanjing Normal University) for technical support.
Funding
This research was financially supported by the National Natural Science Foundation of China (8220102395), the Natural Science Foundation of Jiangsu Province of China(BK20220375), and the Jiangsu provincial colleges of Natural Science General Program(22KJB350007).
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TZ and WZ designed and finished the project, and ZL and WY contributed to the data analysis and manuscript writing. LY and HH assisted in this experiment and helped complete the manuscript revision.
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Tu, Z., Zhou, L., Wu, Y. et al. Construction of prokaryotic nanocompartment in Yarrowia lipolytica to assist phloroglucinol production. Appl Microbiol Biotechnol 107, 5341–5349 (2023). https://doi.org/10.1007/s00253-023-12668-0
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DOI: https://doi.org/10.1007/s00253-023-12668-0