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Evaluation of a bacterial group 1 LEA protein as an enzyme protectant from stress-induced inactivation

  • Biotechnologically Relevant Enzymes and Proteins
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Abstract

Late embryogenesis abundant (LEA) proteins are hydrophilic proteins that lack a well-ordered tertiary structure and accumulate to high levels in response to water deficit, in organisms such as plants, fungi, and bacteria. The mechanisms proposed to protect cellular structures and enzymes are water replacement, ion sequestering, and membrane stabilization. The activity of some proteins has a limited shelf-life due to instability that can be caused by their structure or the presence of a stress condition that limits their activity; several LEA proteins have been shown to behave as cryoprotectants in vitro. Here, we report a group1 LEA from Azotobacter vinelandii AvLEA1, capable of conferring protection to lactate dehydrogenase, catechol dioxygenase, and Baylase peroxidase against freeze-thaw treatments, desiccation, and oxidative damage, making AvLEA a promising biological stabilizer reagent. This is the first evidence of protection provided by this LEA on enzymes with biotechnological potential, such as dioxygenase and peroxidase under in vitro stress conditions. Our results suggest that AvLEA could act as a molecular chaperone, or a “molecular shield,” preventing either dissociation or antiaggregation, or as a radical scavenger, thus preventing damage to these target enzymes during induced stress.

Key points

• This work expands the basic knowledge of the less-known bacterial LEA proteins and their in vitro protection potential.

• AvLEA is a bacterial protein that confers in vitro protection to three enzymes with different characteristics and oligomeric arrangement.

• The use of AvLEA as a stabilizer agent could be further explored using dioxygenase and peroxidase in bioremediation treatments.

Graphical Abstract

AvLEA1 protects against freeze-thaw treatments, desiccation, and oxidative damage on three different enzymes with biotechnological potential.

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Funding

This research was funded by PAPIIT-DGAPA IN207019. LPL received financial support from PASPA DGAPA-UNAM for a sabbatical year.

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Authors and Affiliations

  1. Departamento de Microbiología Molecular, Instituto de Biotecnología, UNAM, Av. Universidad #2001, Col. Chamilpa, 62210, Cuernavaca, Morelos, Mexico

    Enrique Raga-Carbajal, Guadalupe Espin, Julieta Rodríguez-Salazar & Liliana Pardo-López

  2. Departamento de Ingeniería Celular Y Biocatálisis, Instituto de Biotecnología, UNAM, Av. Universidad #2001, Col. Chamilpa, 62210, Cuernavaca, Morelos, Mexico

    Marcela Ayala

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  1. Enrique Raga-Carbajal
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  2. Guadalupe Espin
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  3. Marcela Ayala
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  4. Julieta Rodríguez-Salazar
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  5. Liliana Pardo-López
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Contributions

ER-C, JR-S, and LP-L contributed to the design and implementation of the research. ER-C performed the experiments and analyzed the data. ER-C, JR-S, LP-L EG-E, and MA contributed to the writing of the manuscript and approved the final version.

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Correspondence to Julieta Rodríguez-Salazar or Liliana Pardo-López.

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Raga-Carbajal, E., Espin, G., Ayala, M. et al. Evaluation of a bacterial group 1 LEA protein as an enzyme protectant from stress-induced inactivation. Appl Microbiol Biotechnol 106, 5551–5562 (2022). https://doi.org/10.1007/s00253-022-12080-0

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