Abstract
Cyanobacterial harmful algal blooms are a worldwide problem with substantial adverse effects on the aquatic environment as well as human health. Among the multiple physicochemical and biotic approaches, algicidal bacterium is one of the most promising and eco-friendly ways to control bloom expansion. In this study, Stenotrophomonas sp. KT48 isolated from the pond where cyanobacterial blooms occurred exhibited a strong inhibitory effect on Microcystis aeruginosa. However, the algicidal performance and mechanisms of Stenotrophomonas sp. remain under-documented. To explore the algicidal performance and physiological response againt M. aeruginosa, further works were implemented here. Our results indicated that the algicidal rate of strain KT48 cultured in 1/8 LB medium supplemented with 0.3% starch or glucose was about 30% higher than that in 1/8 LB medium. Strain KT48 culture, cell-free filtrate, and cells re-suspended were inoculated into the M. aeruginosa culture, and the Chl-a content was determined. Those results indicated that the algicidal activity of cells re-suspended was far higher than that of cell-free filtrate and culture. Thus, strain KT48 exhibited algicidal activity mainly through direct attacking M. aeruginosa rather than excretion of algicides. Furthermore, strain KT48 led to an increase in cellular reactive oxygen species (ROS) and caused lipid peroxidation as supported by the increase in malondialdehyde (MDA) levels. The ROS and MDA levels in algal cells treated with strain KT48 cells re-suspended were about 3.23-fold and 2.80-fold higher than those of untreated algal cells on day 11. And a further inhibition to the antioxidant system is suggested by a sharp decrease in the superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) activities. In addition, we also observed that the morphology of most algal cells changed from integrity to break. This study not only indicated strain KT48 with strong algicidal activity, but also explored the underlying algicidal mechanisms to provide a source of bacterial agent for the biocontrol of cyanobacterial blooms.
Key points
• Strain KT48 exhibited strong algicidal activity mainly through direct attacking M. aeruginosa.
• The addition of glucose could enhance the algicidal rate of strain KT48 by about 30%.
• Strain KT48 led to an increase in cellular reactive oxygen species (ROS) level that causes membrane damage as supported by the increase in malondialdehyde (MDA) levels.
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The authors declare that the data supporting the findings of this study are available within the article and its supplementary information files.
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This research was supported by the National Natural Science Foundation of China (No. 91951108, No.21976197, and No.42076155) and the Key R&D plan of Ningxia Hui Autonomous Region (2019BFG02032).
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P.L. conceived and designed the research and wrote the manuscript. S.X and X.Z. refined the experimental method. P.L., X.Z., and Y.Q. performed the experiments. P.L. and H.L. analyzed the data. H.Z., X.Z., and C.W. reviewed and edited the manuscript. X.Z., D.W., and B.X. supervised the project. All authors have read the final manuscript and approved it.
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Lyu, P., Li, H., Zheng, X. et al. Oxidative stress of Microcystis aeruginosa induced by algicidal bacterium Stenotrophomonas sp. KT48. Appl Microbiol Biotechnol 106, 4329–4340 (2022). https://doi.org/10.1007/s00253-022-11959-2
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DOI: https://doi.org/10.1007/s00253-022-11959-2