Abstract
Plantaricin EF, a kind of natural antibacterial substance, has shown inhibitory effect on most pathogen and spoilage microorganisms, which possessed great potential in food preservation. However, the lower production of plantaricin EF has limited its large-scale production and application. In this study, the effect of maltose on plantaricin EF production and its regulation mechanism in Lactobacillus plantarum 163 were investigated. Maltose significantly improved the biomass and plantaricin EF production, which increased by 3.35 and 3.99 times comparing to the control without maltose, respectively. The maximum production of plantaricin E and F in fed-batch fermentation were 10.55 mg/L and 22.94 mg/L, respectively. Besides, qPCR results showed that maltose remarkably improved transcription of plnA, plnB, plnD, plnE, plnF, plnG1 and plnH, and heighten transcription of lamR, lamK, hpk6 and rrp6. These results provided an effective method to enhance plantaricin EF production and revealed a possible regulatory mechanism from transcriptome results that hpk6, rrp6, lamK and lamR were relative to plantaricin EF production. Genes, hpk6 and rrp6, promote transcription of plnG1, whereas lamK and lamR enhance transcription of plnA, plnB and plnD, which increased plantaricin EF production.
Keypoints
• Maltose was proved to be effective in promoting the biosynthesis of plantaricin EF.
• Maltose promoted the transcription of biosynthesis and secretion genes of plantaricin EF.
• Up-regulation of genes lamR, lamK, hpk6 and rrp6 heightened the plantaricin EF production.
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Data availability
The data of whole genome sequence and transcriptome sequence on L. plantarum 163 have been submitted to the Sequence Read Archive database of National Center for Biotechnology Information, and the accession numbers were SRR12825168 and SRR12831476, respectively. Other related data generated or analyzed during this study are included in this published article.
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The authors acknowledge the financial support for this work provided by the National Natural Science Foundation of China (No. 31771948).
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Z.X.L. and Y.J.L. conceived and designed research. D.Y.Z. conducted experiments. F.Q.M., L.B.Z. and J.S. analyzed data. D.Y.Z., F.X.L. and X.M.B. wrote the manuscript. Z.X.L. and Y.J.L. reviewed and edited this manuscript. Z.X.L. is also the supervision, project administration of this manuscript. All authors read and approved the manuscript.
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Zhao, D., Meng, F., Zhou, L. et al. Maltose effective improving production and regulatory biosynthesis of plantaricin EF in Lactobacillus plantarum 163. Appl Microbiol Biotechnol 105, 2713–2723 (2021). https://doi.org/10.1007/s00253-021-11218-w
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DOI: https://doi.org/10.1007/s00253-021-11218-w