Abstract
Cytochrome P450 enzymes (P450s) are one of the major factors responsible for the diversity of metabolites produced through many biosynthetic and biodegradative processes in actinomycetes. P450s typically catalyze a single oxidative modification; however, several P450s have been identified with the unique ability to iteratively oxidize the same-site of the substrate. These P450s are capable of forming diverse compounds that affect biological processes, including alcohols, ketones, aldehydes, and carboxylic acids. Although further structural and functional studies are needed to elucidate the mechanisms that allow multistep oxidative modification, recent studies have revealed the enzymatic properties and reaction mechanisms of these P450s. This mini-review covers the current knowledge of P450s that catalyze the multistep oxidation reactions and contribute to the production of a wide variety of metabolites by selected actinomycete strains, along with insights into their application and utility. Understanding the characteristics of these remarkable enzymes will facilitate their utilization in biotechnological applications to create biologically active and other high-value compounds.
Key points
• The multistep oxidation by P450s plays a key role in the diversity of metabolites.
• The mechanisms that enable P450s to catalyze iterative oxidation remains unknown.
• The effective use of P450s that iteratively oxidize the same-site is discussed.
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This work was supported by JSPS KAKENHI (Grant Number JP20K16034).
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YI designed and drafted this review. YA and DS revised the manuscript. All the authors read and approved the final manuscript.
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Iizaka, Y., Sherman, D.H. & Anzai, Y. An overview of the cytochrome P450 enzymes that catalyze the same-site multistep oxidation reactions in biotechnologically relevant selected actinomycete strains. Appl Microbiol Biotechnol 105, 2647–2661 (2021). https://doi.org/10.1007/s00253-021-11216-y
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DOI: https://doi.org/10.1007/s00253-021-11216-y