Abstract
Colorimetric, fluorescence, and paper-based method were developed to measure the Hg2+ level in water using iturin A, a lipopeptide produced by Bacillus subtilis. Firstly, iturin was used to synthesize highly stable and uniformly sized silver nanoparticles (AgNPs). Secondly, the iturin-AgNPs were found to be highly selective and sensitive to Hg2+. The absorbance of the reaction system showed a good linear correlation with the Hg2+ concentration from 0.5 to 5 mg/L at 450 nm in the UV-Vis spectroscopy detection with the limit of detection (LOD) of 0.5 mg/L. When the reaction system was detected by fluorescence measurement, a good linear relationship was found between the fluorescence intensity and Hg2+ concentration from 0.05 to 0.5 mg/ at 415 nm with the LOD of 0.05 mg/L. Lastly, a paper-based detection method was developed. The developed method was successfully used to detect Hg2+ in contaminated polluted waters and showed acceptable results in terms of sensitivity, selectivity and stability. The paper-based method could distinguish Hg2+ at levels higher than 0.05 mg/L, thereby meeting the guidelines of the effluent quality standard for industries (0.05 mg/L). In summary, this method can be used daily by various industries to monitor the Hg2+ level in effluent water.
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Acknowledgments
We acknowledge funding by the Innovation Foundation for Doctor Dissertation of Northwestern Polytechnical University (CX201929), the Modern Agricultural Industry Technology System (CARS-30), the Key research and development plan of Shaanxi Province (2017ZDXL-NY-0304, 2019ZDLNY01-02-02), the China Postdoctoral Science Foundation (2017 M613211), the Shaanxi Postdoctoral Science Foundation(2017BSHEDZZ119), and the Fundamental Research Funds for the Central Universities (3102018jgc010). We also thank Key Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, Analytical &Testing Center, Northwestern Polytechnical University for providing all instruments used in the study.
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Zhao, X., Ai, C., Li, Z. et al. Development of a paper-based method to detect Hg2+ in waste water using iturin from Bacillus subtilis. Appl Microbiol Biotechnol 103, 8609–8618 (2019). https://doi.org/10.1007/s00253-019-10109-5
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DOI: https://doi.org/10.1007/s00253-019-10109-5