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A comprehensive analysis of Candida albicans phosphoproteome reveals dynamic changes in phosphoprotein abundance during hyphal morphogenesis

  • Genomics, transcriptomics, proteomics
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Abstract

The morphological plasticity of Candida albicans is a virulence determinant as the hyphal form has significant roles in the infection process. Recently, phosphoregulation of proteins through phosphorylation and dephosphorylation events has gained importance in studying the regulation of pathogenicity at the molecular level. To understand the importance of phosphorylation in hyphal morphogenesis, global analysis of the phosphoproteome was performed after hyphal induction with elevated temperature, serum, and N-acetyl-glucosamine (GlcNAc) treatments. The study identified 60, 20, and 53 phosphoproteins unique to elevated temperature-, serum-, and GlcNAc-treated conditions, respectively. Distribution of unique phosphorylation sites sorted by the modified amino acids revealed that predominant phosphorylation occurs in serine, followed by threonine and tyrosine residues in all the datasets. However, the frequency distribution of phosphorylation sites in the proteins varied with treatment conditions. Further, interaction network-based functional annotation of protein kinases of C. albicans as well as identified phosphoproteins was performed, which demonstrated the interaction of kinases with phosphoproteins during filamentous growth. Altogether, the present findings will serve as a base for further functional studies in the aspects of protein kinase-target protein interaction in effectuating phosphorylation of target proteins, and delineating the downstream signaling networks linked to virulence characteristics of C. albicans.

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Acknowledgements

This work is financially supported by grants from Council of Scientific and Industrial Research, Department of Biotechnology and the Core Grant of National Institute of Plant Genome Research, New Delhi, India. P.G. and M.I. acknowledge Council of Scientific and Industrial Research, India, for the Senior Research Fellowship and Senior Research Associateship, respectively.

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Authors and Affiliations

  1. National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi, 110067, India

    Priyanka Ghorai, Mohammad Irfan & Asis Datta

  2. Department of Biotechnology, School of Chemical and Life Sciences, Jamia Hamdard, Hamdard Nagar, New Delhi, 110062, India

    Priyanka Ghorai & Alka Narula

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  1. Priyanka Ghorai
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  2. Mohammad Irfan
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  3. Alka Narula
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  4. Asis Datta
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Contributions

A.D. conceived and supervised the complete study. P.G., M.I., and A.D. planned the experiments. P.G. performed all the experiments. P.G., M.I., A.D., and A.N. analyzed the results and wrote the manuscript. All authors have read and approved the final manuscript.

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Correspondence to Asis Datta.

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The study is financially supported by Council of Scientific and Industrial Research [Grant Number 38(1408/15/Emr-II)], India, Department of Biotechnology (Grant Number BT/101/CFIB/12/II/01), India, and the Core Grant of National Institute of Plant Genome Research, New Delhi, India. This article does not contain any studies with human participants or animals performed by any of the authors.

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The authors declare that they have no conflict of interest.

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Ghorai, P., Irfan, M., Narula, A. et al. A comprehensive analysis of Candida albicans phosphoproteome reveals dynamic changes in phosphoprotein abundance during hyphal morphogenesis. Appl Microbiol Biotechnol 102, 9731–9743 (2018). https://doi.org/10.1007/s00253-018-9303-z

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