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iTRAQ-based proteomic profiling of a Microbacterium sp. strain during benzo(a)pyrene removal under anaerobic conditions

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Abstract

This study focused on the protein expression of a Microbacterium sp. strain that utilized various concentrations of benzo(a)pyrene (BaP) as the sole source of carbon and energy under anaerobic conditions. A total of 1539 protein species were quantified by isobaric tags for relative and absolute quantitation (iTRAQ) coupled with LC-MS/MS. GO, COG, and pathway enrichment analysis showed that most proteins demonstrated catalytic and binding functions and were mainly involved in metabolic processes, cellular processes, and single-organism processes. Sixty-two proteins were found in their abundances in BaP-stress conditions different from normal conditions. These proteins function in the metabolic pathways; the biosynthesis of secondary metabolites, the biosynthesis of antibiotics, microbial metabolism in diverse environments, carbon metabolism, and the biosynthesis of amino acids were markedly altered. Furthermore, enoyl-CoA hydratase was proposed to be a key protein during BaP removal of the Microbacterium sp. strain. This study provides a powerful platform for the further exploration of BaP removal, and the differentially expressed proteins provide insight into the mechanism of the BaP removal pathway.

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Funding

This work was supported by the National Natural Science Foundation of China [grant numbers 51579010] and the Fundamental Research Funds for the Central Universities [grant numbers 2014NT32].

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Authors and Affiliations

  1. College of Water Sciences, Beijing Normal University, Beijing, 100875, People’s Republic of China

    Junfeng Dou, Wei Qin, Aizhong Ding & Yi Zhu

  2. School of Environment, Tsinghua University, Beijing, 100084, People’s Republic of China

    Xiang Liu

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  1. Junfeng Dou
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  2. Wei Qin
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  3. Aizhong Ding
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  4. Xiang Liu
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Correspondence to Yi Zhu.

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Dou, J., Qin, W., Ding, A. et al. iTRAQ-based proteomic profiling of a Microbacterium sp. strain during benzo(a)pyrene removal under anaerobic conditions. Appl Microbiol Biotechnol 101, 8365–8377 (2017). https://doi.org/10.1007/s00253-017-8536-6

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