Abstract
The methylotrophic yeast Pichia pastoris is an attractive expression system due to its ability to secrete large amounts of recombinant protein, with the potential for glycosylation. Advances in glycoengineering of P. pastoris have successfully demonstrated the humanization of both the N- and O-linked glycosylation pathways in this organism. However, in certain cases, the presence of O-linked glycans on a therapeutic protein may not be desirable. Recently, we have reported the in vitro utility of jack bean α-1,2/3/6-mannosidase to remove O-linked mannose from intact undenatured glycoproteins produced in glycoengineered P. pastoris. However, one caveat of this strategy is that jack bean mannosidase has yet to be cloned and as such is only available as crude cellular extracts. This raises several concerns for using this reagent to treat large preparations of therapeutic proteins generated in P. pastoris. Therefore, we postulated that lysosomal mannosidases which have been cloned and demonstrated to have similar activities to jack bean mannosidase on N-linked glycans would also process O-linked glycans in a similar fashion. To this end, we screened a panel of recombinant lysosomal mannosidases from different organisms and identified several which cannot only reduce extended O-linked mannose chains but which can also hydrolyze the Man-α-O-Ser/Thr glycosidic bond on intact glycoproteins. As such, not only do we show for the first time the utility of lysosomal mannosidase for O-linked mannose processing, but since this is a recombinant enzyme, it has several benefits over the use of crude jack bean mannosidase extracts.
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Acknowledgments
The authors would like to thank Dr. Michael Meehl for the P. pastoris produced glycoprotein substrates used in this study, Stephanie Nelson and Nathan Sharkey for their fermentation support, and both Heather Lynaugh and Irina Burnina for their analytical assistance.
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The authors declare that they have no conflict of interest.
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Daniel Hopkins and Sujatha Gomathinayagam contributed equally to this work.
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Hopkins, D., Gomathinayagam, S. & Hamilton, S.R. A practical approach for O-linked mannose removal: the use of recombinant lysosomal mannosidase. Appl Microbiol Biotechnol 99, 3913–3927 (2015). https://doi.org/10.1007/s00253-014-6189-2
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DOI: https://doi.org/10.1007/s00253-014-6189-2