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dsrAB-based analysis of sulphate-reducing bacteria in moving bed biofilm reactor (MBBR) wastewater treatment plants

  • Environmental biotechnology
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Abstract

Sulphate-reducing bacteria (SRB) are important members of the sulphur cycle in wastewater treatment plants (WWTPs). In this study, we investigate the diversity and activity of SRB within the developing and established biofilm of two moving bed biofilm reactor (MBBR) systems treating municipal wastewater in New Zealand. The larger of the two WWTPs (Moa Point) generates high levels of sulphide relative to the smaller Karori plant. Clone libraries of the dissimilatory (bi)sulphite reductase (dsrAB) genes and quantitative real-time PCR targeting dsrA transcripts were used to compare SRB communities between the two WWTPs. Desulfobulbus (35–53 % of total SRB sequences) and genera belonging to the family Desulfobacteraceae (27–41 %) dominated the SRB fraction of the developing biofilm on deployed plastic carriers at both sites, whereas Desulfovibrio and Desulfomicrobium were exclusively found at Moa Point. In contrast, the established biofilms from resident MBBR carriers were largely dominated by Desulfomonile tiedjei-like organisms (58–100 % of SRB sequences). The relative transcript abundance of dsrA genes (signifying active SRBs) increased with biofilm weight yet remained low overall, even in the mature biofilm stage. Our results indicate that although SRB are both present and active in the microbial community at both MBBR study sites, differences in the availability of sulphate may be contributing to the observed differences in sulphide production at these two plants.

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Acknowledgments

The authors would like to thank Veolia Limited for the assistance with this study.

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Authors and Affiliations

  1. Centre for Microbial Innovation, School of Biological Sciences, The University of Auckland, Private Bag 92019, Auckland, 1142, New Zealand

    Kristi Biswas, Michael W. Taylor & Susan J. Turner

  2. BioDiscovery New Zealand Limited, Brooke House, Parnell, Auckland, New Zealand

    Susan J. Turner

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  1. Kristi Biswas
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  2. Michael W. Taylor
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Correspondence to Kristi Biswas.

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Biswas, K., Taylor, M.W. & Turner, S.J. dsrAB-based analysis of sulphate-reducing bacteria in moving bed biofilm reactor (MBBR) wastewater treatment plants. Appl Microbiol Biotechnol 98, 7211–7222 (2014). https://doi.org/10.1007/s00253-014-5769-5

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