Abstract
In the last decade, various large-scale projects describing soil microbial diversity across large geographical gradients have been undertaken. However, many questions remain unanswered about the best ways to conduct these studies. In this review, we present an overview of the experience gathered during these projects, and of the challenges that future projects will face, such as standardization of protocols and results, considering the temporal variation of microbiomes, and the legal constraints limiting such studies. We also present the arguments for and against the exhaustive description of soil microbiomes. Finally, we look at future developments of soil microbiome studies, notably emphasizing the important role of cultivation techniques.
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Acknowledgements
We thank the OCP Africa team for their helpful comments, and Andrew Blakney for the English editing and commenting to this manuscript. We also thank Stéphane Daigle for his advice on statistical mapping.
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This study received funding from the OCP Innovation (Grant Number: AS-85).
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Both authors contributed to the writing of the review. The first draft of the manuscript was written by Jean Legeay, and Mohamed Hijri commented on and modified previous versions of the manuscript. Both authors read and approved the final manuscript.
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Legeay, J., Hijri, M. A Comprehensive Insight of Current and Future Challenges in Large-Scale Soil Microbiome Analyses. Microb Ecol 86, 75–85 (2023). https://doi.org/10.1007/s00248-022-02060-2
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DOI: https://doi.org/10.1007/s00248-022-02060-2