Abstract
The application of road salt as a deicing agent is common, but investigations of potential negative effects of salt runoff on stream salamanders have been limited. Additionally, modern stormwater management practices should influence the delivery of salt ions to streams. We used data loggers in streams draining watersheds with and without stormwater management ponds and acute 96-h LC50 tests to investigate exposure of, and road salt toxicity among, two widespread salamanders in the Eastern USA, northern dusky salamanders (Desmognathus fuscus) and northern two-lined salamanders (Eurycea bislineata). In streams below stormwater ponds, base levels of conductivity were elevated throughout the year and elevated Cl− levels led to more frequent acute and chronic exceedances of U.S. Environmental Protection Agency ambient water quality criteria for Cl− when compared to streams draining watersheds with no stormwater management ponds. However, five of the six streams studied had exceedance frequencies suggesting Cl− associated with road salt application represented a persistent threat to aquatic life. Larval stream salamanders were relatively tolerant of salt, not exhibiting any lethal effects over a 96-h period until chloride levels exceeded 5000 mg/L for both species, and concentrations in streams rarely exceeded these levels and only for very short periods of time. Our results suggest road salts are not having acute lethal effects on salamanders in the streams we studied, but exceedance of U.S. Environmental Protection Agency ambient water quality standards for Cl− suggest the potential for sublethal and indirect effects of Cl− on salamander populations that require further study.
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All data from this study are available upon request to the corresponding author.
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All R code from this study is available upon request to the corresponding author.
References
Albecker MA, McCoy MW (2017) Adaptive responses to salinity stress across multiple life stages in anuran amphibians. Front Zool 14:40. https://doi.org/10.1186/s12983-017-0222-0
Barbier L, Suaire R, Durickovic I, Laurent J, Simonnot M (2018) Is a road stormwater retention pond able to intercept deicing salt? Water. Air Soil Pollut 229:251. https://doi.org/10.1007/s11270-018-3908-9
Barrett K, Price SJ (2014) Urbanization and stream salamanders: a review, conservation options, and research needs. Freshw Sci 33:927–940. https://doi.org/10.1086/677556
Barrett K, Helms BS, Guyer C, Schoonover JE (2010a) Linking process to pattern: causes of stream-breeding amphibian decline in urbanized watersheds. Biol Conserv 143:1998–2005. https://doi.org/10.1016/j.biocon.2010.05.001
Barrett K, Helms BS, Samoray ST, Guyer C (2010b) Growth patterns of a stream vertebrate differ between urban and forested catchments. Freshw Biol 55:1628–1635. https://doi.org/10.1111/j.1365-2427.2009.02393.x
Beckingham B, Callahan T, Vulava V (2019) Stormwater ponds in the Southeastern U.S. Coastal Plain: hydrogeology contaminant fate and the need for a social-ecological framework. Front Environ Sci 7. https://doi.org/10.3389/fenvs.2019.00117
Bird DL, Groffman PM, Salice CJ, Moore J (2018) Steady-state land cover but non-steady-state major ion chemistry in urban streams. Environ Sci Technol 52:13015–13026. https://doi.org/10.1021/acs.est.8b03587
Cañedo-Argüelles M, Hawkins CP, Kefford BJ, Schäfer RB, Dyack BJ, Brucet S, Buchwalter D, Dunlop J, Frör O, Lazorchak J, Coring E, Fernandez HR, Goodfellow W, Achem ALG, Hatfield-Dodds S, Karimov BK, Mensah P, Olson JR, Piscart C, Prat N, Timpano AJ et al (2016) Saving freshwater from salts. Science 351:914–916. https://doi.org/10.1126/science.aad3488
Casey RE, Lev SM, Snodgrass JW (2012) Stormwater ponds as a source of long-term surface and ground water salinisation. Urban Water J 10:1–9. https://doi.org/10.1080/1573062X.2012.716070
Chapra SC, Dove A, Rockwell DC (2009) Great Lakes chloride trends: long-term mass balance and loading analysis. J Great Lakes Res 35:272–284. https://doi.org/10.1016/j.jglr.2008.11.013
Collins SJ, Russell RW (2009) Toxicity of road salt to Nova Scotia amphibians. Environ Pollut 157:320–324. https://doi.org/10.1016/j.envpol.2008.06.032
Cooper CA, Mayer PM, Faulkner BR (2014) Effects of road salts on groundwater and surface water dynamics of sodium and chloride in an urban restored stream. Biogeochemistry 121:149–166. https://doi.org/10.1007/s10533-014-9968-z
Corsi SR, De Cicco LA, Lutz MA, Hirsch RM (2015) River chloride trends in snow-affected urban watersheds: Increasing concentrations outpace urban growth rate and are common among all seasons. Sci Total Environ 508:488–497. https://doi.org/10.1016/j.scitotenv.2014.12.012
Dalinsky SA, Lolya LM, Maguder JL, Pierce JLB, Kelting DL, Laxson CL, Patrick DA (2014) Comparing the effects of aquatic stressors on model temperate freshwater aquatic communities. Water Air Soil Pollut 225:2007. https://doi.org/10.1007/s11270-014-2007-9
Dananay KL, Krynak KL, Krynak TJ, Benard MF (2015) Legacy of road salt: apparent positive larval effects counteracted by negative post-metamorphic effects in wood frogs. Environ Toxicol Chem 34:2417–2424. https://doi.org/10.1002/etc.3082
Dugan HA, Bartlett SL, Burke SM, Doubek JP, Krivak-Tetley FE, Skaff NK, Summers JC, Farrell KJ, McCullough IM, Morales-Williams AM, Roberts DC, Ouyang Z, Scordo F, Hanson PC, Weathers KC (2017) Salting our freshwater lakes. Proc Natl Acad Sci USA 114:4453–4458. https://doi.org/10.1073/pnas.1620211114
Dunson WA (1977) Tolerance to high temperature and salinity by tadpoles of the Philippine frog, Rana cancrivora. Copeia 1977:375–378. https://doi.org/10.2307/1443921
Environment Canada and Health Canada (2001) Priority substances list assessment report−road salt. En40–215/63E
Gallagher MT, Snodgrass JW, Brand AB, Casey RE, Lev SM, Van Meter RJ (2014) The role of pollutant accumulation in determining the use of stormwater ponds by amphibians. Wetl Ecol Manag 22:551–554. https://doi.org/10.1007/s11273-014-9351-9
Hamilton MA, Russo RC, Thurston RV (1977) Trimmed Spearman-Karber methods for estimating median lethal concentrations in toxicity bioassays. Environ Sci Technol 11:714–719
Harless ML, Huckins CJ, Grant JB, Pypker TG (2011) Effects of six chemical deicers on larval wood frogs (Rana sylvatica). Environ Toxicol Chem 30:1637–1641. https://doi.org/10.1002/etc.544
Hill AR, Sadowski EK (2016) Chloride concentrations in wetlands along a rural to urban land use gradient. Wetlands 36:73–83. https://doi.org/10.1007/s13157-015-0717-4
Hopkins GR, Brodie ED (2015) Occurrence of amphibians in saline habitats: a review and evolutionary Perspective. Herpetol Monogr 29:1–27. https://doi.org/10.1655/HERPMONOGRAPHS-D-14-00006
Howard KWF, Haynes J (1993) Groundwater contamination due to road de-icing chemicals−salt balance implications. Geosci Canada 20:1–8
Hubbart JA, Kellner E, Hooper LW, Zeiger S (2017) Quantifying loading, toxic concentrations, and systemic persistence of chloride in a contemporary mixed-land-use watershed using an experimental watershed approach. Sci Total Environ 581–582:822–832. https://doi.org/10.1016/j.scitotenv.2017.01.019
Jackson RB, Jobbágy EG (2005) From icy roads to salty streams. Proc Natl Acad Sci USA 102:14487–14488. https://doi.org/10.1073/pnas.0507389102
Jones B, Snodgrass JW, Ownby DR (2015) Relative toxicity of NaCl and road deicing salt to developing amphibians. Copeia 103:72–77. https://doi.org/10.1643/CP-13-082
Jones DK, Mattes BM, Hintz WD, Schuler MS, Stoler AB, Lind LA, Cooper RO, Relyea RA (2017) Investigation of road salts and biotic stressors on freshwater wetland communities. Environ Pollut 221:159–167. https://doi.org/10.1016/j.envpol.2016.11.060
Karraker NE, Gibbs JP, Vonesh JR (2008) Impacts of road deicing salt on the demography of vernal pool-breeding amphibians. Ecol Appl 18:724–734. https://doi.org/10.1890/07-1644.1
Kaushal SS, Groffman PM, Likens GE, Belt KT, Stack WP, Kelly VR, Band LE, Fisher GT (2005) Increased salinization of fresh water in the northeastern United States. Proc Natl Acad Sci USA 102:13517–13520. https://doi.org/10.1073/pnas.0506414102
Kaushal SS, Duan S, Doody TR, Haq S, Smith RM, Newcomer Johnson TA, Newcomb KD, Gorman J, Bowman N, Mayer PM, Wood KL, Belt KT, Stack WP (2017) Human-accelerated weathering increases salinization, major ions, and alkalinization in fresh water across land use. Appl Geochem 83:121–135. https://doi.org/10.1016/j.apgeochem.2017.02.006
Kaushal SS, Likens GE, Pace ML, Utz RM, Haq S, Gorman J, Grese M (2018) Freshwater salinization syndrome on a continental scale. Proc Natl Acad Sci USA. https://doi.org/10.1073/pnas.1711234115
Kefford BJ, Papas PJ, Metzeling L, Nugegoda D (2004) Do laboratory salinity tolerances of freshwater animals correspond with their field salinity? Environ Pollut 129:355–362. https://doi.org/10.1016/j.envpol.2003.12.005
Kelly VR, Lovett GM, Weathers KC, Findlay SEG, Strayer DL, Burns DJ, Likens GE (2008) Long-term sodium chloride retention in a rural watershed: legacy effects of road salt on streamwater concentration. Environ Sci Technol 42:410–415. https://doi.org/10.1021/es071391l
Mahajan CL, Sharma SD, Sharma SP (1979) Tolerance of aquatic organisms to chloride salts. Indian J Exp Biol 17:1244–1245
Meriano M, Eyles N, Howard KWF (2009) Hydrogeological impacts of road salt from Canada’s busiest highway on a Lake Ontario watershed (Frenchman’s Bay) and lagoon, City of Pickering. J Contam Hydrol 107:66–81. https://doi.org/10.1016/j.jconhyd.2009.04.002
Moore J, Fanelli RM, Sekelick AJ (2020) High-frequency data reveal deicing salts drive elevated specific conductance and chloride along with pervasive and frequent exceedances of U.S. environmental protection agency aquatic life criteria for chloride in urban streams. Environ Sci Technol 54:778–789. https://doi.org/10.1021/acs.est.9b04316
Murphy MO, Agha M, Maigret TA, Price SJ, Dorcas ME (2016) The effects of urbanization on body size of larval stream salamanders. Urban Ecosyst 19:275–286. https://doi.org/10.1007/s11252-015-0486-0
Novotny EV, Murphy D, Stefan HG (2008) Increase of urban lake salinity by road deicing salt. Sci Total Environ 406:131–144. https://doi.org/10.1016/j.scitotenv.2008.07.037
Orser P, Shure D (1972) Effects of urbanization on the salamander Desmognathus fuscus fuscus. Ecology 53:1148–1154. https://doi.org/10.2307/1935428
Padhye AD, Ghate HV (1992) Sodium chloride and potassium chloride tolerance of different stages of the frog, Microhyla ornata. Herpetol J 2:18–23
Paul MJ, Meyer JL (2001) Streams in the urban landscape. Annu Rev Ecol Syst 32:333–365. https://doi.org/10.1146/annurev.ecolsys.32.081501.114040
Price SJ, Cecala KK, Browne RA, Dorcas ME (2011) Effects of urbanization on occupancy of stream salamanders. Conserv Biol 25:547–555. https://doi.org/10.1111/j.1523-1739.2010.01627.x
Price SJ, Browne RA, Dorcas ME (2012) Evaluating the effects of urbanisation on salamander abundances using a before-after control-impact design. Freshw Biol 57:193–203. https://doi.org/10.1111/j.1365-2427.2011.02699.x
R Core Team (2021) R: A language and environment for statistical computing. R foundation for statistical computing, Vienna, Austria. http://www.R-project.org/. Version 4.0.5
Rizzo AA, Raesly RL, Hilderbrand RR (2015) Stream salamander responses to varying degrees of urbanization within Maryland’s piedmont physiographic province. Urban Ecosyst 19:397–413. https://doi.org/10.1007/s11252-015-0504-2
Roy JW (2019) Endobenthic organisms exposed to chronically high chloride from groundwater discharging along freshwater urban streams and lakeshores. Environ Sci Technol 53:9389–9397. https://doi.org/10.1021/acs.est.9b02288
Roy JW, Bickerton G (2012) Toxic groundwater contaminants: an overlooked contributor to urban stream syndrome? Environ Sci Technol 46:729–736. https://doi.org/10.1021/es2034137
Santos B, Ribeiro R, Domingues I, Pereira R, Soares AMVM, Lopes I (2013) Salinity and copper interactive effects on Perez’s frog Pelophylax perezi. Environ Toxicol Chem 32:1864–1872. https://doi.org/10.1002/etc.2257
Sanzo D, Hecnar SJ (2006) Effects of road de-icing salt (NaCl) on larval wood frogs (Rana sylvatica). Environ Pollut 140:247–256. https://doi.org/10.1016/j.envpol.2005.07.013
Skidds DE, Golet FC (2005) Estimating hydroperiod suitability for breeding amphibians in southern Rhode Island seasonal forest ponds. Wetl Ecol Manag 13:349–366. https://doi.org/10.1007/s11273-004-7527-4
Snodgrass JW, Moore J, Lev SM, Casey RE, Ownby DR, Flora RF, Izzo G (2017) Influence of modern stormwater management practices on transport of road salt to surface waters. Environ Sci Technol 51:4165–4172. https://doi.org/10.1021/acs.est.6b03107
Thunqvist E (2004) Regional increase of mean chloride concentration in water due to the application of deicing salt. Sci Total Environ 325:29–37. https://doi.org/10.1016/j.scitotenv.2003.11.020
Trowbridge PR, Kahl JS, Sassan DA, Heath DL, Walsh EM (2010) Relating road salt to exceedances of the water quality standard for chloride in new Hampshire streams. Environ Sci Technol 44:4903–4909. https://doi.org/10.1021/es100325j
U.S. EPA (1988) Ambient water quality for chloride. U.S. Environmental Protection Agency, Office of Water Criteria and Standards, Washington D.C., EPA 440/5–88–001
U.S. EPA (2002) Short-term methods for estimating the chronic toxicity of effluents and receiving waters to freshwater organisms. Fourth Edition. U.S. Environmental Protection Agency, Office of Water, EPA-821-R-02–013
Van Meter RJ, Swan CM, Leips J, Snodgrass JW (2011) Road salt stress induces novel food web structure and interactions. Wetlands 31:843–851. https://doi.org/10.1007/s13157-011-0199-y
Viertel B (1999) Salt tolerance of Rana temporaria: Spawning site selection and survival during embryonic development (Amphibia, Anura). Amphib Reptil 20:161–171. https://doi.org/10.1163/156853899X00178
Walsh CJ, Roy AH, Feminella JW, Cottingham PD, Groffman PM, Morgan RP (2005) The urban stream syndrome: current knowledge and the search for a cure. J North Am Benthol Soc 24:706–723. https://doi.org/10.1899/04-028.1
Williams WD (2001) Anthropogenic salinisation of inland waters. Hydrobiologia 466:329–337. https://doi.org/10.1007/978-94-017-2934-5_30
Willson JD, Dorcas ME (2003) Effects of habitat disturbance on stream salamanders: implications for buffer zones and watershed management. Conserv Biol 17:763–771. https://doi.org/10.1046/j.1523-1739.2003.02069.x
Yang L, Jin S, Danielson P, Homer CG, Gass L, Bender SM, Case A, Costello C, Dewitz JA, Fry JA, Funk M, Granneman BJ, Liknes GC, Rigge MB, Xian G (2018) A new generation of the United States National land cover database—requirements, research priorities, design, and implementation strategies: ISPRS. J Photogramm Rem S 146:108–123. https://doi.org/10.1016/j.isprsjprs.2018.09.006
Zeileis A, Grothendieck G (2005) zoo: S3 infrastructure for regular and irregular time series. J Stat Softw 14:1–27
Acknowledgements
This project was funded by Towson University, Fisher College of Science and Mathematics, and Towson University Graduate Student Association. Ryan Casey provided input on study design and Mark Monk assisted with ion chromatography analysis. All procedures and handling of amphibians was approved by the Towson University IACUC (protocol # SP0809RPR.03).
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All authors contributed to the study conception and design. Material preparation, data collection, and laboratory analyses were carried out by GI. Statistical analyses were performed by GI and JWS. The first draft of the manuscript was written by GI and JWS, and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.
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Izzo, G., Ownby, D. & Snodgrass, J.W. Stream Salamanders are Relatively Tolerant of Salty Streams. Arch Environ Contam Toxicol 82, 255–265 (2022). https://doi.org/10.1007/s00244-021-00875-7
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DOI: https://doi.org/10.1007/s00244-021-00875-7