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Identification of ACE inhibitory peptides from red alga Mazzaella japonica

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Abstract

Angiotensin I converting enzyme (ACE) inhibition is one of the key factors for cardiovascular disease, and inhibition of ACE activity is related to the prevention of high blood pressure. Until now, some ACE inhibitory peptides have been found in the protein hydrolysates of red algae, and most of them were derived from phycobiliproteins and rubisco. In this study, we modified the preparation method to evaluate the potential of ACE inhibitory activity from protein hydrolysate of red alga Mazzaella japonica except for phycobiliproteins and rubisco. As a result, we identified 11 peptides (YRD, VSEGLD, TIMPHPR, GGPAT, SSNDYPI, SRIYNVKSNG, VDAHY, CPYDWV, YGDPDHY, NLGN and DFGVPGHEP) in the hydrolysate by the reversed phase-HPLC and MALDI-TOF/MS/MS. Among them, YRD would be derived from phycoerythrin α-subunit. However, the others would be from other proteins encoded in chloroplast or nuclear genomes. This study revealed that not only phycobiliproteins and rubisco but also the other proteins in red algae have the potential for the source of ACE inhibitory peptide.

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Acknowledgements

This study was partially supported by the “Science and technology research promotion program for agriculture, forestry, fisheries and food industry (27004B)”.

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Authors and Affiliations

  1. Laboratory of Marine Chemical Resource Development, Faculty of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido, 041-8611, Japan

    Yuya Kumagai & Hideki Kishimura

  2. Chair of Marine Chemical Resource Development, Graduate School of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido, 041-8611, Japan

    Yumi Kitade, Manami Kobayashi, Kei Watanabe & Hiroki Kurita

  3. Hokkaido Research Organization, Fisheries Research Department, Abashiri Fisheries Research Institute, 7-8-5 Minato-cho Monbetsu, Hokkaido, 094-0011, Japan

    Hirohumi Takeda

  4. Laboratory of Humans and the Ocean, Faculty of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido, 041-8611, Japan

    Hajime Yasui

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  1. Yuya Kumagai
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  2. Yumi Kitade
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  3. Manami Kobayashi
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  8. Hideki Kishimura
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Correspondence to Hideki Kishimura.

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Kumagai, Y., Kitade, Y., Kobayashi, M. et al. Identification of ACE inhibitory peptides from red alga Mazzaella japonica. Eur Food Res Technol 246, 2225–2231 (2020). https://doi.org/10.1007/s00217-020-03567-z

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  • DOI: https://doi.org/10.1007/s00217-020-03567-z

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