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Purification and identification of dipeptidyl peptidase IV and angiotensin-converting enzyme inhibitory peptides from silver carp (Hypophthalmichthys molitrix) muscle hydrolysate

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Abstract

Diabetes and hypertension are increasing threats to human health, which could be improved by inhibiting dipeptidyl peptidase IV (DPP-IV) and angiotensin-converting enzyme (ACE), respectively. Herein, we used five different enzymes to hydrolyze silver carp (Hypophthalmichthys molitrix) muscle protein. Among all treatments, the Neutrase-generated hydrolysate that was purified by a three-step isolation and then was identified using liquid chromatography–tandem mass spectrometry resulted in the most potent DPP-IV inhibitory activity and relatively high ACE inhibitory activity. The identified peptide Ala-Ala-Leu-Glu-Gln-Thr-Glu-Arg was the most effective at inhibiting DPP-IV with a half-maximal inhibitory concentration (IC50) value of 647.02 ± 4.30 µM. Leu-Leu-Asp-Leu-Gly-Val-Pro showed the highest ACE inhibitory activity with an IC50 value of 329.33 ± 15.53 µM. Lys-Ala-Val-Gly-Glu-Pro-Pro-Leu-Phe exhibited the dual inhibition against both DPP-IV and ACE with IC50 values of 1,317.39 ± 20.93 µM and 726.01 ± 8.69 µM, respectively. Interestingly, it seemed that the majority of peptides which were responsible for inhibiting DPP-IV were different from the ones which caused the inhibition of ACE.

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Acknowledgements

This study was supported by the National Science and Technology Ministry of China (Award number 2017YFD0400200) and the China Agriculture Research System (CARS-45).

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Authors and Affiliations

  1. College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, 10083, China

    Yuqi Zhang, Hui Hong & Yongkang Luo

  2. Beijing Guotai Biotechnology Co., Ltd, Beijing, 100011, China

    Huaigao Liu

  3. Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB, T6G 2P5, Canada

    Hui Hong

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Correspondence to Yongkang Luo.

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Zhang, Y., Liu, H., Hong, H. et al. Purification and identification of dipeptidyl peptidase IV and angiotensin-converting enzyme inhibitory peptides from silver carp (Hypophthalmichthys molitrix) muscle hydrolysate. Eur Food Res Technol 245, 243–255 (2019). https://doi.org/10.1007/s00217-018-3157-6

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  • DOI: https://doi.org/10.1007/s00217-018-3157-6

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