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Characterization and geographical discrimination of saffron from Greece, Spain, Iran, and Morocco based on volatile and bioactivity markers, using chemometrics

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Abstract

In the present study saffron samples from Greece, Spain, Iran, and Morocco, collected during harvesting years 2005–2014, were characterized and classified according to geographical origin using volatile compounds and selected bioactivity parameters. The volatile compounds of saffron were semi-quantified using headspace solid-phase microextraction in combination with gas chromatography/mass spectrometry. Bioactivity parameters such as total phenolic content (TPC), radical scavenging activity (%RSA), and ferric reducing antioxidant power (FRAP) were determined using spectrophotometric assays. Results showed that significant variations (P < 0.05) in the volatile content, TPC, and antioxidant activity were observed according to geographical origin of saffron. Certain volatile compounds and bioactivity parameters were also affected by harvesting year (P < 0.05). Finally, strong positive Pearson’s correlations were obtained for TPC and %RSA (r = 0.90), TPC and FRAP (r = 0.90), highlighting that the methanolic fraction of saffron is rich in phytochemicals, contributing thus, to its overall antioxidant properties. The application of LDA to above combined parameters determined, enabled the correct geographical discrimination of saffron, resulting in a correct prediction rate of 95.5%. The use of a larger number of saffron samples will further validate present results.

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Acknowledgements

The authors are grateful to saffron cooperative ‘Krokos Kozanis’ for the donation of Greek saffron samples, and to Dr. Arantzazu Valdés and Dr. Ali Khanjari, for the collection of saffron samples from Spain and Iran, respectively. Excellent technical assistance of Mr. Christos Nikolaou is also greatly acknowledged.

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Authors and Affiliations

  1. Laboratory of Food Chemistry, Department of Chemistry, University of Ioannina, Ioannina, 45110, Greece

    Ioannis K. Karabagias, Marina Koutsoumpou, Vasiliki Liakou & Michael G. Kontominas

  2. Department of Social Administration and Political Science, Democritus University of Thrace, Komotini, 69100, Greece

    Stavros Kontakos

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  1. Ioannis K. Karabagias
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  2. Marina Koutsoumpou
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  3. Vasiliki Liakou
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  4. Stavros Kontakos
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  5. Michael G. Kontominas
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Correspondence to Ioannis K. Karabagias or Michael G. Kontominas.

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Karabagias, I.K., Koutsoumpou, M., Liakou, V. et al. Characterization and geographical discrimination of saffron from Greece, Spain, Iran, and Morocco based on volatile and bioactivity markers, using chemometrics. Eur Food Res Technol 243, 1577–1591 (2017). https://doi.org/10.1007/s00217-017-2866-6

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  • DOI: https://doi.org/10.1007/s00217-017-2866-6

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