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Preparation, characterization and bioactivity of xylobiose and xylotriose from corncob xylan by xylanase

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Abstract

Xylo-oligosaccharides, which are usually produced from xylan by enzymatic hydrolysis, have already become newly developed functional oligosaccharides with many beneficial biomedical and health effects. Xylo-oligosaccharides prepared by enzymatic hydrolysis from xylan were analyzed by thin-layer chromatography and high-pressure liquid chromatography in this study. Furthermore, xylose, xylobiose and xylotriose were separated by silica gel column chromatography. Then, their structures were identified by electrospray ionization mass spectrometry and nuclear magnetic resonance. Experiment promoting the discrepancy in bifidobacterium proliferation showed that xylotriose processed the best, followed by xylobiose and xylo-oligosaccharide. So, the main active ingredients of xylo-oligosaccharides were xylobiose and xylotriose, and the best bifidogenic factor was xylotriose.

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Acknowledgments

This work was financially supported by the Foundation of Tianjin University of Science and Technology (Nos. 20120106), the Foundation of Tianjin Educational Committee (Nos. 20090604) and National Spark Program of China (2012GA610005).

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None.

Compliance with Ethics Requirements

This article does not contain any studies with human or animal subjects.

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Authors and Affiliations

  1. Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Science and Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, People’s Republic of China

    Zhen-Yuan Zhu, Liang Zhao, Xiao-Ran Ge, Ya-Li Tang & Wei Pang

  2. Key Laboratory of Freshwater Fishery Germplasm Resources, Ministry of Agriculture, Shanghai Ocean University, Shanghai, 200090, People’s Republic of China

    Li-Jing Chen

  3. UPMC Univ Paris 06, CNRS, UMR 8232, IPCM, Sorbonne Universités, 4 place Jussieu, 75005, Paris, France

    Yongmin Zhang

Authors
  1. Zhen-Yuan Zhu
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  2. Liang Zhao
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  3. Xiao-Ran Ge
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  4. Ya-Li Tang
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  5. Li-Jing Chen
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  6. Wei Pang
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  7. Yongmin Zhang
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Correspondence to Zhen-Yuan Zhu or Li-Jing Chen.

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Zhu, ZY., Zhao, L., Ge, XR. et al. Preparation, characterization and bioactivity of xylobiose and xylotriose from corncob xylan by xylanase. Eur Food Res Technol 241, 27–35 (2015). https://doi.org/10.1007/s00217-015-2431-0

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  • DOI: https://doi.org/10.1007/s00217-015-2431-0

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