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Polymer-enhanced peroxidase activity of ceria nanozyme for highly sensitive detection of alkaline phosphatase

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Abstract

Nanoceria have demonstrated a wide array of catalytic activity similar to natural enzymes, holding considerable significance in the colorimetric detection of alkaline phosphatase (ALP), which is a biomarker of various biological disorders. However, the issues of physiological stability and formation of protein corona, which are strongly related to their surface chemistry, limit their practical application. In this work, CeO2 nanoparticles characterized by enhanced dimensional uniformity and specific surface area were synthesized, followed by encapsulation with various polymers to further increase catalytic activity and physiological stability. Notably, the CeO2 nanoparticles encapsulated within each polymer exhibited improved catalytic characteristics, with PAA-capped CeO2 exhibiting the highest performance. We further demonstrated that the PAA-CeO2 obtained with enhanced catalytic activity was attributed to an increase in surface negative charge. PAA-CeO2 enabled the quantitative assessment of AA activity within a wide concentration range of 10 to 60 μM, with a detection limit of 0.111 μM. Similarly, it allowed for the evaluation of alkaline phosphatase activity throughout a broad range of 10 to 80 U/L, with a detection limit of 0.12 U/L. These detection limits provided adequate sensitivity for the practical detection of ALP in human serum.

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Acknowledgements

This work was financially supported by GDAS Project of Science and Technology Development (2022GDASZH-2022010101), Natural Science Foundation of China (Nos. 21603067, 22073025), and Research and Development Plan in Key Areas of Guangdong Province (No. 2022B1111040002).

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Author notes

  1. Qian Wang and Song Meng contributed equally to this work.

Authors and Affiliations

  1. Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou, 510070, People’s Republic of China

    Qian Wang, Gang Zhou, Qingshan Shi & Xiaobao Xie

  2. National & Local Joint Engineering Research Center of High-Throughput Drug Screening Technology, College of Health Science and Engineering, School of Materials Science & Engineering, Hubei University, Wuhan, 430062, China

    Song Meng & Ziqiang Xu

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  1. Qian Wang
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Contributions

Wang Qian: Conceptualization, Data curation, Writing - Original draft, Writing - Reviewing and editing.

Meng Song: Investigation, Methodology.

Zhou Gang: Funding acquisition, Investigation.

Shi Qingshan: Formal analysis.

Xu Ziqiang: Project administration, Data curation.

Xie Xiaobao: Supervision, Validation.

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Correspondence to Ziqiang Xu or Xiaobao Xie.

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Published in the topical collection featuring Nanozymes with guest editors Vipul Bansal, Sudipta Seal, and Hui Wei.

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Wang, Q., Meng, S., Zhou, G. et al. Polymer-enhanced peroxidase activity of ceria nanozyme for highly sensitive detection of alkaline phosphatase. Anal Bioanal Chem (2024). https://doi.org/10.1007/s00216-024-05307-8

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