Abstract
The detection of phytohormones in real time has attracted increasing attention because of their critical roles in regulating the development and signaling of plants, especially in defense against biotic stresses. Herein, stainless steel sheet electrodes modified with carbon cement were coupled with paper-based analysis devices for direct and simultaneous detection of salicylic acid (SA) and indole-3-acetic acid (IAA) in plants. We demonstrated that the excellent conductivity of stainless steel sheet electrodes enabled us to simultaneously differentiate IAA and SA at a level of 10 nM. With our approach, the content of IAA and SA in Arabidopsis thaliana leaves infected or not infected with Pst DC3000 could be rapidly quantified at the same time. Our experimental results on differentiation of IAA and SA at different time points showed that there were antagonistic interactions between the IAA and SA after infection of Arabidopsis leaves with Pst DC3000. By offering a cost-effective approach for rapid and sensitive detection of IAA and SA, this study suggests that electrochemical detection can be used in the study and development of precision agriculture technology.
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Acknowledgements
This work was supported by the National Natural Science Foundation of China (Nos: 31770399, 21974012 and 32070397), the Natural Science Foundation of Jiangsu Province (Nos: BK20130389), the project of Nantong Natural Science Foundation (MS22021038), Qing Lan Project of Jiangsu Province, Six talent peaks project in Jiangsu Province (No: SWYY-061), and the science and technology innovation project of Jiangsu Province (202110304080Y).
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Conceptualization, Lijun Sun and Ning Bao; Methodology, Ling Sun, Songzhi Xu, and Yihui Tang; Validation, Yihui Tang; Formal analysis, Ling Sun, Yuhang Zhou and Meng Wang; Investigation, Xinyu Zhu; Data curation, Guangxi Li and Yuhang Zhou; Writing-original draft preparation, Ling Sun, Songzhi Xu and Lijun Sun; Writing-review and editing, Songzhi Xu, Lijun Sun and Ning Bao; Visualization, Xinyu Zhu and Yiran Tian; Supervision and project administration, Lijun Sun; Funding acquisition, Lijun Sun. All authors have read and agreed to the published version of the manuscript.
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Sun, L., Xu, S., Tang, Y. et al. Disposable stainless steel working electrodes for sensitive and simultaneous detection of indole-3-acetic acid and salicylic acid in Arabidopsis thaliana leaves under biotic stresses. Anal Bioanal Chem 414, 7721–7730 (2022). https://doi.org/10.1007/s00216-022-04303-0
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DOI: https://doi.org/10.1007/s00216-022-04303-0