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A NIR fluorescence probe for monitoring Cys upregulation induced by balsam pear polysaccharide and imaging in zebrafish

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Abstract

In this work, we introduced the acrylate recognition group into dicyanoisophorone derivative DCI-C–OH to construct the NIR fluorescent probe DCI-C-Cys with a large Stokes shift (240 nm). DCI-C-Cys could specifically respond to Cys, resulting in a 22-fold increase in fluorescence intensity at 702 nm. Meanwhile, the probe has the advantages of good water solubility, high sensitivity (93 nM), and excellent biocompatibility. Moreover, DCI-C-Cys successfully monitored endogenous and exogenous Cys in HepG2 cells and zebrafish. Most importantly, we found that balsam pear polysaccharide could lead to the increase of intracellular Cys levels, which might be conducive to the further study of the antioxidant mechanism of balsam pear polysaccharide.

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Funding

This work was supported by the Natural Science Foundation of Henan Province (No. 212300410222, 202300410327); Science & Technology Project for Young Talents of Henan Province (No. 2022HYTP042); Excellent Youth Science Foundation of Henan Province (No. 202300410310); the Key Scientific Research Foundation of the Higher Education Institutions of Henan Province (No. 21A310009); the Key Project of Science and Technology of Henan Province (No. 192102310122); and the Development Program for Key Young Teachers in Colleges and Universities of Henan Province (No. 2020GGJS-146).

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  1. Qiaofang Qi and Cuiling Shang contributed equally to this work.

Authors and Affiliations

  1. School of Basic Medical Sciences, Xinxiang Medical University, Xinxiang, 453003, China

    Qiaofang Qi, Huayu Wang, Chunpo Ge,  ZhijunYang, Tianjun Ni & Kaiwen Chang

  2. The Third Affiliated Hospital of Xinxiang Medical University, Xinxiang, 453003, China

    Cuiling Shang

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  1. Qiaofang Qi
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Qi, Q., Shang, C., Wang, H. et al. A NIR fluorescence probe for monitoring Cys upregulation induced by balsam pear polysaccharide and imaging in zebrafish. Anal Bioanal Chem 414, 6871–6880 (2022). https://doi.org/10.1007/s00216-022-04252-8

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