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Electrical potential-assisted DNA-RNA hybridization for rapid microRNA extraction

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Abstract

Analysis of microRNAs (miRNAs) is important in cancer diagnostics and therapy. Conventional methods used to extract miRNA for analysis are generally time-consuming. A novel approach for rapid and sensitive extraction of miRNAs is urgently need for clinical applications. Herein, a novel strategy based on electrical potential-assisted DNA-RNA hybridization was designed for miRNA extraction. The entire extraction process was accomplished in approximately 3 min, which is much shorter than the commercial adsorption column method, at more than 60 min, or the TRIzol method, at more than 90 min. Additionally, the method offered the advantages of simplicity and specificity during the extraction process by electrical potential-assisted hybridization of single-stranded DNA and RNA. Taking let-7a as an example, satisfactory results were achieved for miRNA extraction in serum, demonstrating the applicability in miRNA nucleic acid amplification.

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Data availability

All data generated or analyzed during this study are included in this manuscript and its supplementary information files.

Abbreviations

miRNAs:

microRNAs

RT-qPCR:

Reverse transcription quantitative polymerase chain reaction

ASEA:

Accelerated strand exchange amplification

TCEP:

Tris(2-carboxyethyl)phosphine hydrochloride

MCH:

6-mercaptohexanol

CV:

Cyclic voltammetry

EIS:

Electrochemical impedance spectroscopy

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Acknowledgements

The study was supported by grants from the National Key Research and Development Programs of China (2018YFE0113300), National Natural Science Foundation of China (81801264) and Key Project of Shandong Provincial Natural Science Foundation (ZR2020KH030).

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Authors and Affiliations

  1. Shandong Provincial Key Laboratory of Biochemical Engineering, Qingdao Nucleic Acid Rapid Detection Engineering Research Center, College of Marine Science and Biological Engineering, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Shandong, 266042, Qingdao, People’s Republic of China

    Xiaoli Zhao, Yong Li, Ritong Sun, Yaofang Fan & Cuiping Ma

  2. Clinical Laboratory, Qingdao Central Hospital, The Second Affiliated Hospital of Medical College of Qingdao University, Qingdao, 266042, China

    Xiaofeng Mu & Ye Wang

  3. Qingdao Nucleic Acid Rapid Testing International Science and Technology Cooperation Base, College of Life Sciences, Department of Pathogenic Biology, School of Basic Medicine, the Clinical Laboratory Department of the Affiliated Hospital of Qingdao University, Qingdao University, Qingdao, 266071, People’s Republic of China

    Chao Shi

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  1. Xiaoli Zhao
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Correspondence to Cuiping Ma.

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Zhao, X., Li, Y., Sun, R. et al. Electrical potential-assisted DNA-RNA hybridization for rapid microRNA extraction. Anal Bioanal Chem 414, 3529–3539 (2022). https://doi.org/10.1007/s00216-022-03979-8

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