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A non-enzymatic urine glucose sensor with 2-D photonic crystal hydrogel

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Abstract

A novel polymerized crystalline colloidal array (PCCA) sensing material for the detection of urine glucose was developed by embedding a two-dimensional (2-D) polystyrene crystalline colloidal array (CCA) in 3-acrylamidophenylboronic acid (3-APBA)-functionalized hydrogel. After adjusting the cross-linker concentration, this material showed significant sensitivity for glucose under lab conditions, the particle spacing of the PCCA changed from 917 to 824 nm (93 nm) within 3 min as the glucose concentration increased from 0 to 10 mM, and the structural color of the PCCA changed from red through orange, to green, and finally, to cyan. In further experiments, this material was used to semi-quantitatively detect glucose in 20 human urine (HU) samples. Compared with the traditional dry-chemistry method, which was applied widely in clinical diagnosis, the PCCA method was more accurate and cost-effective. Moreover, this method can efficiently avoid the errors induced by most of the urine-interfering elements like vitamin C and ketone body. With a homemade portable optical detector, this low-cost intelligent sensing material can provide a more convenient and efficient strategy for the urine glucose detection in clinical diagnosis and point-of-care monitoring.

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Acknowledgments

The financial supports from the National Natural Science Foundation of China (NSFC) (21375009 and U1530141) are appreciated.

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Authors and Affiliations

  1. School of Chemical Engineering and Environment, Beijing Institute of Technology, Zhongguancun South Street 5, Beijing, 100081, China

    Zequn Yan, Min Xue, Wei Lu, Zihui Meng, Dan Yan, Lili Qiu, Lijun Zhou & Yingjie Yu

  2. Department of Clinical Laboratory, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shanxi, 710004, China

    Qian He

Authors
  1. Zequn Yan
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  2. Min Xue
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  3. Qian He
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  4. Wei Lu
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  5. Zihui Meng
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  6. Dan Yan
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  7. Lili Qiu
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  8. Lijun Zhou
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  9. Yingjie Yu
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Corresponding authors

Correspondence to Zihui Meng or Lili Qiu.

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All participants provided written informed consent for themselves after having received a complete description of the study, which was approved by the Ethics Committee of The Second Affiliated Hospital of Xi’an Jiaotong University, Shanxi.

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The authors declare that they have no conflict of interest.

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Yan, Z., Xue, M., He, Q. et al. A non-enzymatic urine glucose sensor with 2-D photonic crystal hydrogel. Anal Bioanal Chem 408, 8317–8323 (2016). https://doi.org/10.1007/s00216-016-9947-4

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  • DOI: https://doi.org/10.1007/s00216-016-9947-4

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