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Faraday cage-type electrochemiluminescence immunosensor for ultrasensitive detection of Vibrio vulnificus based on multi-functionalized graphene oxide

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Abstract

A novel Faraday cage-type electrochemiluminescence (ECL) immunosensor devoted to the detection of Vibrio vulnificus (VV) was fabricated. The sensing strategy was presented by a unique Faraday cage-type immunocomplex based on immunomagnetic beads (IMBs) and multi-functionalized graphene oxide (GO) labeled with (2,2′-bipyridine)(5-aminophenanthroline)ruthenium (Ru-NH2). The multi-functionalized GO could sit on the electrode surface directly due to the large surface area, abundant functional groups, and good electronic transport property. It ensures that more Ru-NH2 is entirely caged and become “effective,” thus improving sensitivity significantly, which resembles extending the outer Helmholtz plane (OHP) of the electrode. Under optimal conditions, the developed immunosensor achieves a limit of detection as low as 1 CFU/mL. Additionally, the proposed immunosensor with high sensitivity and selectivity can be used for the detection of real samples. The novel Faraday cage-type method has shown potential application for the diagnosis of VV and opens up a new avenue in ECL immunoassay.

Faraday cage-type immunoassay mode for ultrasensitive detection by extending OHP

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Abbreviations

Ab1 :

Anti-VV1

Ab2 :

Anti-VV2

APW:

Alkaline peptone water

BSA:

Bovine serum albumin

EC:

Enterobacter cloacae

ECL:

Electrochemiluminescence

ECLIA:

Electrochemiluminescence immunoassay

EDC:

1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride

EIS:

Electrochemical impedance spectroscopy

ELISA:

Enzyme-linked immunosorbent assay

GLD:

Glutaraldehyde

GO:

Graphene oxide

IHP:

Interior Helmholtz plane

IMBs:

Immunomagnetic beads

LAMP:

Loop-mediated isothermal amplification

MGCE:

Modified magnetic glass carbon electrode

MPN:

Most probable number

NHS:

N-Hydroxysuccinimide

OHP:

Outer Helmholtz plane

PCR:

Polymerase chain reaction

Ru-NH2 :

(2,2′-Bipyridine)(5-aminophenanthroline)ruthenium

SM:

Shewanella marisflavi

TPrA:

Tripropylamine

VH:

Vibrio harveyi

VP:

Vibrio parahaemolyticus

VV:

Vibrio vulnificus

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Authors and Affiliations

  1. Faculty of Materials Science and Chemical Engineering, Ningbo University, Ningbo, Zhèjiāng, 315211, China

    Zhiyong Guo, Yuhong Sha, Yufang Hu, Zhongqing Yu, Yingying Tao, Yanjie Wu, Min Zeng, Sui Wang & Xing Li

  2. School of Marine Sciences, Ningbo University, Ningbo, Zhèjiāng, 315211, China

    Jun Zhou & Xiurong Su

Authors
  1. Zhiyong Guo
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  2. Yuhong Sha
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  3. Yufang Hu
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  7. Min Zeng
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  10. Jun Zhou
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Corresponding authors

Correspondence to Zhiyong Guo or Xiurong Su.

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Funding

Financial supports from the National Natural Science Foundation of China (81273130, 41576098, 21571110), State Oceanic Administration of the People’s Republic of China (201105007), and Science and Technology Department of Zhejiang Province of China (2016C33176) are gratefully acknowledged. This work was also sponsored by K.C. Wong Magna Fund in Ningbo University.

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The authors declare that they have no conflict of interest.

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Published in the topical collection Analytical Electrochemiluminescence with guest editors Hua Cui, Francesco Paolucci, Neso Sojic, and Guobao Xu.

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Guo, Z., Sha, Y., Hu, Y. et al. Faraday cage-type electrochemiluminescence immunosensor for ultrasensitive detection of Vibrio vulnificus based on multi-functionalized graphene oxide. Anal Bioanal Chem 408, 7203–7211 (2016). https://doi.org/10.1007/s00216-016-9851-y

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