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Determination of illicit drugs and metabolites in oral fluid by microextraction on packed sorbent coupled with LC-MS/MS

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Abstract

Oral fluid (OF) has become a valuable biologic specimen for toxicological analysis, especially in driving under the influence of drugs (DUID) investigations, because of easy and non-invasive collection procedures. In OF testing, being the sample volume is limited, multi-analyte procedures are particularly advantageous since they save time and resources. In this work, a procedure for the simultaneous analysis of 20 illicit drugs, belonging to the classes of cocaine, amphetamines, natural and synthetic opioids and hallucinogens, is presented. The sample preparation is based on microextraction by packed sorbent (MEPS), a novel technique which is based on the miniaturization of solid-phase extraction (SPE). The presented method, which includes all the most diffused illicit drugs and their metabolites, has been fully validated according to the Scientific Working Group for Forensic Toxicology (SWGTOX) guidelines. LLOQs ranged from 0.5 to 30 ng mL−1 (diacetylmorphine); the presented method allows the detection of all the selected drugs quite below the cutoff values recommended by Substance Abuse and Mental Health Services Administration (SAMHSA) for abuse identification.

In the present work a procedure for the simultaneous quantification of 20 illicit drugs, belonging to different classes is presented. The sample preparation is based on microextraction by packed sorbent (MEPS), followed by LC-MS/MS analysis

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Author information

Authors and Affiliations

  1. Department of Chemistry, Sapienza University of Rome, 00185, Rome, Italy

    Camilla Montesano, Maria Chiara Simeoni & Roberta Curini

  2. Faculty of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, 64023, Mosciano Sant’Angelo, TE, Italy

    Maria Chiara Simeoni, Manuel Sergi, Claudio Lo Sterzo & Dario Compagnone

Authors
  1. Camilla Montesano
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  2. Maria Chiara Simeoni
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  3. Roberta Curini
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  4. Manuel Sergi
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  5. Claudio Lo Sterzo
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  6. Dario Compagnone
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Correspondence to Manuel Sergi.

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Montesano, C., Simeoni, M.C., Curini, R. et al. Determination of illicit drugs and metabolites in oral fluid by microextraction on packed sorbent coupled with LC-MS/MS. Anal Bioanal Chem 407, 3647–3658 (2015). https://doi.org/10.1007/s00216-015-8583-8

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  • DOI: https://doi.org/10.1007/s00216-015-8583-8

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