Abstract
The gut microbiome is involved in the pathogenesis of many diseases including polycystic ovarian syndrome (PCOS). Modulating the gut microbiome can lead to eubiosis and treatment of various metabolic conditions. However, there is no proper study assessing the delivery of microbial technology for the treatment of such conditions. The present study involves the development of guar gum-pectin-based solid self-nanoemulsifying drug delivery system (S-SNEDDS) containing curcumin (CCM) and fecal microbiota extract (FME) for the treatment of PCOS. The optimized S-SNEDDS containing FME and CCM was prepared by dissolving CCM (25 mg) in an isotropic mixture consisting of Labrafil M 1944 CS, Transcutol P, and Tween-80 and solidified using lactose monohydrate, aerosil-200, guar gum, and pectin (colon-targeted CCM solid self-nanoemulsifying drug delivery system [CCM-CT-S-SNEDDS]). Pharmacokinetic and pharmacodynamic evaluation was carried out on letrozole-induced female Wistar rats. The results of pharmacokinetic studies indicated about 13.11 and 23.48-fold increase in AUC of CCM-loaded colon-targeted S-SNEDDS without FME (CCM-CT-S-SNEDDS (WFME)) and CCM-loaded colon-targeted S-SNEDDS with FME [(CCM-CT-S-SNEDDS (FME)) as compared to unprocessed CCM. The pharmacodynamic study indicated excellent recovery/reversal in the rats treated with CCM-CT-S-SNEDDS low and high dose containing FME (group 13 and group 14) in a dose-dependent manner. The developed formulation showcasing its improved bioavailability, targeted action, and therapeutic activity in ameliorating PCOS can be utilized as an adjuvant therapy for developing a dosage form, scale-up, and technology transfer.
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Leander Corrie: methodology, data curation, and writing—original draft
Hardeep Singh: methodology
Monica Gulati: supervision and writing—review and editing
Sukriti Vishwas: methodology
Dinesh Kumar Chellappan: methodology
Gaurav Gupta: methodology
Ana Cláudia Paiva-Santos: methodology, writing—review and editing
Francisco Veiga: methodology, writing—review and editing
Faisal Alotaibi: methodology
Aftab Alam: methodology, writing—review and editing
Rajaraman D. Eri: writing—review and editing, methodology
Parteek Prasher: methodology
Jon Adams: methodology
Keshav Raj Paudel: methodology
Kamal Dua: methodology
Sachin Kumar Singh: methodology, supervision, and writing—review and editing
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Corrie, L., Singh, H., Gulati, M. et al. Polysaccharide-fecal microbiota-based colon-targeted self-nanoemulsifying drug delivery system of curcumin for treating polycystic ovarian syndrome. Naunyn-Schmiedeberg's Arch Pharmacol (2024). https://doi.org/10.1007/s00210-024-03029-3
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DOI: https://doi.org/10.1007/s00210-024-03029-3