Abstract
One Gram stain-positive, catalase-negative, α-hemolytic, chain-forming or paired cocci, designated ST22-14T, was isolated from a blood culture of a child with suspected infection. The results of 16S rRNA gene sequences analyses showed that the most closely related species to strain ST22-14T were "Streptococcus vulneris" DM3B3T (99.2%), Streptococcus mitis NCTC 12261T (99.0%), "Streptococcus gwangjuense" ChDC B345T, (99.0%), Streptococcus oralis subsp. dentisani 7747T (99.0%), Streptococcus downii CECT 9732T (99.0%), and Streptococcus infantis ATCC 700779T (98.9%). The genome of strain ST22-14T consists of 2,053,261 bp with a G + C content of 39.4%. Average nucleotide identity values between strain ST22-14T and Streptococcus mitis NCTC 12261T or other five species were from 82.2 to 88.0%. In silico DNA–DNA hybridization of ST22-14T showed an estimated DNA reassociation value of 34.6% with the closest species. The main cellular fatty acids of strain ST22-14T were 16:0, 18:0, 14:0, 18:1ω7c and 18:1ω6c. Based on these results, strain ST22-14T should be classified as a novel species of genus Streptococcus, for which the name Streptococcus taonis sp. nov. is proposed (type strain ST22-14T = NBRC 116002T = BCRC 81402T).
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References
Ali L, Spiess M, Wobser D, Rodriguez M, Blum HE, Sakιnç T (2016) Identification and functional characterization of the putative polysaccharide biosynthesis protein (CapD) of Enterococcus faecium U0317. Infect Genet Evol 37:215–224. https://doi.org/10.1016/j.meegid.2015.11.020
Andrewes FW, Horder TJ (1906) A study of the Streptococcui pathogenic for man. Lancet 168:708–713. https://doi.org/10.1016/S0140-6736(01)31538-6
Arbique JC et al (2004) Accuracy of phenotypic and genotypic testing for identification of Streptococcus pneumoniae and description of Streptococcus pseudopneumoniae sp. nov. J Clin Microbiol 42:4686–4696. https://doi.org/10.1128/jcm.42.10.4686-4696.2004
Bankevich A et al (2012) SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing. J Comput Biol 19:455–477. https://doi.org/10.1089/cmb.2012.0021
Belman S, Chaguza C, Kumar N, Lo S, Bentley SD (2022) A new perspective on ancient Mitis group streptococcal genetics. Microb Genom. https://doi.org/10.1099/mgen.0.000753
Bentley RW, Leigh JA, Collins MD (1991) Intrageneric structure of Streptococcus based on comparative analysis of small-subunit rRNA sequences. Int J Syst Bacteriol 41:487–494. https://doi.org/10.1099/00207713-41-4-487
Blue CE, Paterson GK, Kerr AR, Bergé M, Claverys JP, Mitchell TJ (2003) ZmpB, a novel virulence factor of Streptococcus pneumoniae that induces tumor necrosis factor alpha production in the respiratory tract. Infect Immun 71:4925–4935. https://doi.org/10.1128/iai.71.9.4925-4935.2003
Bridge PD, Sneath PHA (1982) Streptococcus gallinarum sp. nov. and Streptococcus oralis sp. nov. Int J Syst Evol Microbiol 32:410–415. https://doi.org/10.1099/00207713-32-4-410
Bukowski M, Wladyka B, Dubin G (2010) Exfoliative toxins of Staphylococcus aureus. Toxins (basel) 2:1148–1165. https://doi.org/10.3390/toxins2051148
Chor B, Hendy MD, Snir S (2006) Maximum likelihood Jukes–Cantor triplets: analytic solutions. Mol Biol Evol 23:626–632. https://doi.org/10.1093/molbev/msj069
Chung JH et al (2022) Streptococcus vulneris sp. nov., isolated from wound of patient with diabetic foot ulcer (DFU). Arch Microbiol 204:359. https://doi.org/10.1007/s00203-022-02981-z
Colaco CMG et al (2023) Pneumococcal bacteraemia in adults over a 10-year period (2011–2020): a clinical and serotype analysis. Intern Med J. https://doi.org/10.1111/imj.16045
Facklam R (2002) What happened to the streptococci: overview of taxonomic and nomenclature changes. Clin Microbiol Rev 15:613–630. https://doi.org/10.1128/cmr.15.4.613-630.2002
Goris J, Konstantinidis KT, Klappenbach JA, Coenye T, Vandamme P, Tiedje JM (2007) DNA-DNA hybridization values and their relationship to whole-genome sequence similarities. Int J Syst Evol Microbiol 57:81–91. https://doi.org/10.1099/ijs.0.64483-0
Han XY, Kamana M, Rolston KV (2006) Viridans streptococci isolated by culture from blood of cancer patients: clinical and microbiologic analysis of 50 cases. J Clin Microbiol 44:160–165. https://doi.org/10.1128/jcm.44.1.160-165.2006
Huang RC, Chiang TT, Chiang MH, Yang YS (2021) First case report of persistent Streptococcus mitis bacteremia caused by prosthesis-induced esophageal rupture. J Formos Med Assoc 120:1274–1275. https://doi.org/10.1016/j.jfma.2020.10.014
Jensen A, Scholz CFP, Kilian M (2016) Re-evaluation of the taxonomy of the Mitis group of the genus Streptococcus based on whole genome phylogenetic analyses, and proposed reclassification of Streptococcus dentisani as Streptococcus oralis subsp. dentisani comb. nov., Streptococcus tigurinus as Streptococcus oralis subsp. tigurinus comb. nov., and Streptococcus oligofermentans as a later synonym of Streptococcus cristatus. Int J Syst Evol Microbiol 66:4803–4820. https://doi.org/10.1099/ijsem.0.001433
Kawamura Y, Hou XG, Sultana F, Miura H, Ezaki T (1995) Determination of 16S rRNA sequences of Streptococcus mitis and Streptococcus gordonii and phylogenetic relationships among members of the genus Streptococcus. Int J Syst Bacteriol 45:406–408. https://doi.org/10.1099/00207713-45-2-406
Kawamura Y et al (1998) Streptococcus peroris sp. nov. and Streptococcus infantis sp. nov., new members of the Streptococcus mitis group, isolated from human clinical specimens. Int J Syst Bacteriol 48(Pt 3):921–927. https://doi.org/10.1099/00207713-48-3-921
Kimura M (1980) A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120. https://doi.org/10.1007/bf01731581
Klimke W et al (2009) The national center for biotechnology information’s protein clusters database. Nucleic Acids Res 37:D216-223. https://doi.org/10.1093/nar/gkn734
Kozaki M, Uchimura T, Okada S (1992) Experimental manual of lactic acid bacteria. Asakurasyoten, Tokyo
Kumar S, Stecher G, Li M, Knyaz C, Tamura K (2018) MEGA X: molecular evolutionary genetics analysis across computing platforms. Mol Biol Evol 35:1547–1549. https://doi.org/10.1093/molbev/msy096
Ladhani S, Joannou CL, Lochrie DP, Evans RW, Poston SM (1999) Clinical, microbial, and biochemical aspects of the exfoliative toxins causing staphylococcal scalded-skin syndrome. Clin Microbiol Rev 12:224–242. https://doi.org/10.1128/cmr.12.2.224
Lee I, Ouk Kim Y, Park SC, Chun J (2016) OrthoANI: an improved algorithm and software for calculating average nucleotide identity. Int J Syst Evol Microbiol 66:1100–1103. https://doi.org/10.1099/ijsem.0.000760
Lopardo HA et al (2022) Beta-lactam antibiotics and viridans group streptococci. Rev Argent Microbiol 54:335–343. https://doi.org/10.1016/j.ram.2022.06.004
Marquart ME (2021) Pathogenicity and virulence of Streptococcus pneumoniae: cutting to the chase on proteases. Virulence 12:766–787. https://doi.org/10.1080/21505594.2021.1889812
Martínez-Lamas L, Limeres-Posse J, Diz-Dios P, Álvarez-Fernández M (2020) Streptococcus downii sp. nov., isolated from the oral cavity of a teenager with Down syndrome. Int J Syst Evol Microbiol 70:4098–4104. https://doi.org/10.1099/ijsem.0.004180
Meier-Kolthoff JP, Auch AF, Klenk HP, Göker M (2013) Genome sequence-based species delimitation with confidence intervals and improved distance functions. BMC Bioinform 14:60. https://doi.org/10.1186/1471-2105-14-60
Na SI, Kim YO, Yoon SH, Ha SM, Baek I, Chun J (2018) UBCG: up-to-date bacterial core gene set and pipeline for phylogenomic tree reconstruction. J Microbiol 56:280–285. https://doi.org/10.1007/s12275-018-8014-6
Nahhal SB, Sarkis P, Zakhem AE, Arnaout MS, Bizri AR (2023) Streptococcus oralis pulmonic valve endocarditis: a case report and review of the literature. J Med Case Rep 17:120. https://doi.org/10.1186/s13256-023-03835-y
Naser SM et al (2005) Application of multilocus sequence analysis (MLSA) for rapid identification of Enterococcus species based on rpoA and pheS genes. Microbiology (reading) 151:2141–2150. https://doi.org/10.1099/mic.0.27840-0
Nomoto R, Kagawa H, Yoshida T (2008) Partial sequencing of sodA gene and its application to identification of Streptococcus dysgalactiae subsp. dysgalactiae isolated from farmed fish. Lett Appl Microbiol 46:95–100. https://doi.org/10.1111/j.1472-765X.2007.02272.x
Overbeek R et al (2014) The SEED and the Rapid Annotation of microbial genomes using Subsystems Technology (RAST). Nucleic Acids Res 42:D206–D214
Park SN et al (2019) Streptococcus gwangjuense sp. nov., isolated from human pericoronitis. Curr Microbiol 76:799–803. https://doi.org/10.1007/s00284-019-01687-8
Rosselló-Móra R, Amann R (2015) Past and future species definitions for Bacteria and Archaea. Syst Appl Microbiol 38:209–216. https://doi.org/10.1016/j.syapm.2015.02.001
Rzhetsky A, Nei M (1992) A simple method for estimating and testing minimum-evolution trees. Mol Biol Evol 9:945–945. https://doi.org/10.1093/oxfordjournals.molbev.a040771
Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425. https://doi.org/10.1093/oxfordjournals.molbev.a040454
Sasser M (1990) MIDI technical note 101. Identification of bacteria by gas chromatography of cellular fatty acids. MIDI, Newark, pp 1–7
Singh N, Poggensee L, Huang Y, Evans CT, Suda KJ, Bulman ZP (2022) Antibiotic susceptibility patterns of viridans group streptococci isolates in the United States from 2010 to 2020. JAC Antimicrob Resist 4:dlac049. https://doi.org/10.1093/jacamr/dlac049
Smith A, Jackson MS, Kennedy H (2004) Antimicrobial susceptibility of viridans group streptococcal blood isolates to eight antimicrobial agents. Scand J Infect Dis 36:259–263. https://doi.org/10.1080/00365540410019435
Tamura K, Nei M (1993) Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Mol Biol Evol 10:512–526. https://doi.org/10.1093/oxfordjournals.molbev.a040023
Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882. https://doi.org/10.1093/nar/25.24.4876
Van Hove S, Werion A, Anantharajah A, Belkhir L, van Dievoet MA, Hantson P (2023) Streptococcus pneumoniae bacteremia with acute kidney injury and transient ADAMTS13 deficiency. Case Rep Infect Dis 2023:3283606. https://doi.org/10.1155/2023/3283606
Willcox MD, Zhu H, Knox KW (2001) Streptococcus australis sp. nov., a novel oral streptococcus. Int J Syst Evol Microbiol 51:1277–1281. https://doi.org/10.1099/00207713-51-4-1277
Zheng W et al (2016) StreptoBase: an oral Streptococcus mitis group genomic resource and analysis platform. PLoS ONE 11:e0151908. https://doi.org/10.1371/journal.pone.0151908
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This study was supported by Taoyuan Hospital, Ministry of Health and Welfare (Grant No. PTH111042).
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Chien-Yu Lee and Chin-Kan Chan designed the work. Yi-Sheng Chen and Hui-Chung Wu wrote the main manuscript text. Momoko Chida, Mika Miyashita, Yun-Shien Lee and Wen-Ting Lin performed the genomic and biochemical analyses. Yu-Chung Chang prepared all figures.
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In this work, we did not access the medical records of the patient. Patient consent was not required since the data presented in this study do not relate to any specific person or persons. This study was approved by the Medical Ethics and Institutional Review Board of Taoyuan General Hospital, Ministry of Health and Welfare (IRB NO. TYGH111016).
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The EMBL/GenBank/DDBJ accession numbers for the 16S rRNA is LC752654 and the accession number for the Whole Genome Shotgun project is JARZZP000000000. The type stain of Streptococcus taonis sp. nov. is strain ST22-14T = BCRC 81402T (Bioresource Collection and Research Centre, Taiwan) = NBRC 116002T (Biological Resource Center, NITE, Japan).
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Lee, CY., Chan, CK., Chida, M. et al. Streptococcus taonis sp. nov., a novel bacterial species isolated from a blood culture of a patient. Arch Microbiol 206, 168 (2024). https://doi.org/10.1007/s00203-024-03884-x
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DOI: https://doi.org/10.1007/s00203-024-03884-x