Abstract
Lipid accumulation in microalgae can be substantially enhanced by exposing the microalgae to abiotic stress, thus increasing biofuel production. However, this also generates reactive oxygen species (ROS), which disrupts cell metabolism and reduces their productivity. Previous mRNA sequencing analyses in Neopyropia yezoensis and its associated microorganisms elucidated a putative glutathione peroxidase (PuGPx) gene. Here, this putative glutathione peroxidase was overexpressed in the microalga Chlamydomonas reinhardtii, which increased cell growth and survival rates compared to the control group under abiotic stress. Additionally, increased lipid accumulation was observed under salinity stress, high-temperature stress, and hydrogen peroxide (H2O2)-induced oxidative stress. These results suggest that PuGPx plays a protective role against abiotic stress in C. reinhardtii and stimulates lipid accumulation, which could be considered advantageous in terms of biofuel production.
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Data Availability
Sequences will be available under the following NCBI GenBank accession numbers: Bacteroidia (MBP7261321.1), Sphingomonadales (MBM3920284.1), Flavobacteriaceae (MBK5209038.1), Chitinophagaceae (MCF8292247.1), and Lewinellaceae (MCB9313222.1).
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Funding
This work was supported by a National Research Foundation of Korea (NRF) grant funded by the Korean government (MSIT) (NRF-2018R1D1A1B07049359) and the National Institute of Fisheries Science, Ministry of Oceans and Fisheries, Korea (R2023022).
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EJP and YC designed the study and carried out the sample collection. JHK carried out experiments and conducted the data analysis and wrote the final version of the manuscript. All authors read and approved the final version of the manuscript.
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Communicated by Yusuf Akhter.
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Kim, J.H., Park, EJ. & Choi, Ji. Overexpression of putative glutathione peroxidase from Neopyropia-associated microorganisms in Chlamydomonas to respond to abiotic stress. Arch Microbiol 205, 163 (2023). https://doi.org/10.1007/s00203-023-03507-x
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DOI: https://doi.org/10.1007/s00203-023-03507-x