Abstract
A novel actinobacterium, designated strain HDS12T, was isolated from fruits collected from Changde city located in the northwest of Hunan Province, China and characterized using a polyphasic approach. The 16S rRNA gene sequence analysis indicated that strain HDS12T belonged to the genus Nocardiopsis, and had highest similarities to N. dassonvillei subsp. dassonvillei CGMCC 4.1231T (99.79%), N. deserti H13T (99.73%), N. alborubida NBRC 13392T (99.66%), N. dassonvillei subsp. crassaminis D1T (99.64%), N. synnemataformans DSM 44143T (99.45%), N. lucentensis DSM 44048T (99.04%), N. aegyptia DSM 44442T (98.90%), N. flavescens CGMCC 4.5723T (98.76%), N. alba DSM 43377T (98.69%) and N. halotolerans DSM 44410T (98.63%), respectively. Phylogenetic analysis based on 16S rRNA gene sequence showed that strain HDS12T formed an independent subclade, suggesting that strain HDS12T could belong to a potential novel species. Phylogenomic analysis demonstrated that strain HDS12T was closely related to N. dassonvillei subsp. dassonvillei CGMCC 4.1231T and N. dassonvillei subsp. crassaminis D1T. However, the average nucleotide identity value and the digital DNA-DNA hybridization value between them were well below 95–96% and 70% cut-off point recommended for delineating species. Based on its phenotypic and chemotaxonomic characteristics, strain HDS12T (= MCCC 1K06173T = JCM 34708T) represents the type strain of a novel species, for which the name Nocardiopsis akebiae sp. nov. is proposed.
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Abbreviations
- MCCC:
-
Marine Culture Collection of China
- ISP:
-
International Streptomyces Project
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Acknowledgements
The authors thank Shuang Wan and Wangjie Xu (Wuhan Benagen Technology Co., Ltd, Hubei, China), Li Gu and Yongpeng Yang (Marine Culture Collection of China, MCCC, Xiamen, China) for providing excellent technical assistance.
Funding
This work was supported by Education Department of Hunan Province (21C0515), Natural Science Foundation of Hunan Province (2021JJ50024), Key projects of Hunan Provincial Department of Education (19A340), the Education department of Hunan province in China (21A0418), the Doctoral Start-up project of Hunan University of Arts and Science (21BSQD09) and Innovation Team of Microbial Technology in Hunan University of Arts and Science (202026).
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WZ and JG conceived the idea of the study. PM and JZ wrote the main manuscript text. JZ, FZ and Jiang He prepared figures and tables. JH, WZ and JG corrected and reviewed the paper. All authors read and approved the manuscript.
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Communicated by Erko Stackebrandt.
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The GenBank/EMBL/DDBJ accession numbers for the full-length 16S rRNA gene sequence and genome sequence of strain HDS12T are OM368592 and CP074132, respectively.
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Mo, P., Zhou, J., Zhou, F. et al. Nocardiopsis akebiae sp. nov., a novel endophytic actinomycete isolated from fruits of Akebia trifoliata. Arch Microbiol 204, 643 (2022). https://doi.org/10.1007/s00203-022-03252-7
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DOI: https://doi.org/10.1007/s00203-022-03252-7