Abstract
Spontaneous production of E colicins is known to occur in only a small fraction of colicinogenic population. The current study aimed to determine if the same holds true for the production of colicin E9 in real time, by investigating the induction dynamics of the promoter of the ColE9 operon which results in the expression of the ColE9 activity and functional genes. A novel fluorescent reporter was constructed which carries the fusion of the ColE9 promoter and the gfpmut2 gene in a low copy number plasmid that was compatible with the native ColE9-J plasmid. Using the fluorescent reporter construct in the non colicinogenic E. coli cells, the induction of the ColE9 promoter was investigated. The current study demonstrates that the spontaneous induction of the ColE9 promoter occurs in a heterogenous manner and this heterogeneity is maintained in a bacterial population for several generations suggesting that it is unlikely due to any irreversible mutation in the bacterial culture. Furthermore, the same investigations were repeated using the colicin E9 producing E. coli cells. Flow cytometry analysis revealed that 7.1 ± 0.68% of the colicin E9 producing E. coli cells expressed GFP albeit only 2.45 ± 0.30% was observed from non colicinogenic E. coli cells. The considerable increase in the number of the fluorescent cells was likely due to the DNase activity of colicin E9 produced by their clonemates, resulting the auto-induction, which can be abolished with the inactivation of the DNase activity of the colicin E9.
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Acknowledgements
Authors are thankful to Professor Uri Alon, Department of Molecular Cell Biology, Weizmann Institute of Science, Israel for provision of pUA66. Authors are also thankful to Higher Education commission, Pakistan for the award of a foreign scholarship to SB.
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SB, CNP and RJ conceived and designed research. SB and MV conducted experiments. CNP contributed to analytical tools. SB and SAT analyzed data. SB, MV and SAT wrote the manuscript. All authors have read and approved the manuscript.
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Bano, S., Vankemmelbeke, M., Penfold, C.N. et al. Heterogeneity in the spontaneous induction of the promoter of the ColE9 operon in Escherichia coli. Arch Microbiol 204, 628 (2022). https://doi.org/10.1007/s00203-022-03242-9
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DOI: https://doi.org/10.1007/s00203-022-03242-9