Abstract
The study aims to select potent bacterial antagonists to be used as biocontrol agents against rhizome rot disease in turmeric (Curcuma longa L.). A total of 48 bacterial isolates were isolated from the rhizosphere of turmeric. These isolates were screened for their in vitro antagonism against Fusarium solani FS-01 and Pythium aphanidermatum (ITCC 7908). Production of volatile organic compounds and chitinase activity were also performed. Among the tested isolates, two bacterial isolates (IJ2 and IJ10) showed the highest inhibitory activity against these fungal pathogens. GC/MS analysis of the crude extract produced by Pseudomonas sp. IJ2 and B. subtilis IJ10 was found to contain many bioactive compounds with antifungal and antimicrobial activities. The rhizome treatment with these isolates exhibited the lowest percent disease severity with high biocontrol efficacy against the tested pathogens. These isolates with promising antagonistic potential, therefore, can be used as biocontrol agents against rhizome rot in turmeric.
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Data availability
The data sequences generated during the study have been deposited in GenBank database (NCBI) under the following accession numbers OP415385, MT193010, MT219296, MT192804, MT192880, OP737784, OP737786, OP737816 and OP737818. (https://www.ncbi.nlm.nih.gov/).
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The first author is thankful to University Grants Commission, India, under the NET-JRF Scheme for the financial support.
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The work was not specifically funded by any agency but the first author was supported financially by University Grants Commission (UGC), India, in the form of a fellowship (NET-JRF Scheme).
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FK performed sample collection, laboratory experiments, data analysis, and writing of the manuscript. HK supervised the research work and experimental design. All authors read and approved the manuscript.
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Kharshandi, F., Kayang, H. Antagonistic potential of rhizobacterial isolates against fungal pathogens causing rhizome rot in turmeric. Arch Microbiol 205, 221 (2023). https://doi.org/10.1007/s00203-023-03565-1
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DOI: https://doi.org/10.1007/s00203-023-03565-1