Abstract
A rod-shaped, Gram-negative staining strain, FBM22T, was isolated from a microbial fermentation bed substrate from a pig farm. Its colonies appeared yellow and were 0.5–1.2 mm in diameter. Cells were 0.3–0.5 μm wide, 0.5–0.83 μm long. Optimal growth occurred at 30 °C and pH 7.0–8.0; NaCl was not required for growth. The strain performed denitrification and nitrate reduction functions. And it could produce catalase. FBM22-1T utilized the following organic substrates for growth: tyrosine, glutamic acid, d-glucose, and galactose. The novel isolate could degrade 2-nitropropane as carbon and nitrogen source. The dominant respiratory quinone was Q-10. The major polar lipids were diphosphatidylglycerol, phosphatidylcholine and phosphatidylethanolamine. C18:1 ω7c, C16:1 ω7c and/ or C16:1 ω6c, and C14:0 2-OH were the major (≥ 8%) fatty acids. The G+C content was 56.8 mol%. FBM22T was found to be a member of the genus Sphingopyxis in the family Sphingomonadaceae of the class Alphaproteobacteria. It had the highest sequence similarity with the type strains Sphingopyxis terrae subsp. ummariensis UI2T (96.47%) and Sphingopyxis terrae subsp. terrae NBRC 15098T (96.40%). Furthermore, FBM22T had 18.7% and 18.4% relatedness (based on digital DNA–DNA hybridization) with its two relatives (S. terrae subsp. ummariensis UI2T and S. terrae subsp. terrae NBRC 15098T). The morphological, physiological, and genotypic differences identified in this study support the classification of FBM22T as a novel species within the genus Sphingopyxis, for which the name Sphingopyxis yananensis sp. nov. is proposed. The type strain is FBM22T (= KCTC 82290T = CCTC AB2020286T).
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Abbreviations
- ANI:
-
Average nucleotide identity
- dDDH:
-
Digital DNA–DNA hybridization
- HPLC:
-
High-performance liquid chromatography
- TLC:
-
Thin-layer chromatography
- SEM:
-
Scanning electron microscope
- NJ:
-
Neighbor-joining
- ML:
-
Maximum-likelihood
- MP:
-
Maximum-parsimony
- GL:
-
Glycolipid
- PL:
-
Unknown phospholipids
- DPG:
-
Diphosphatidylglycerol
- PC:
-
Phosphatidylcholine
- PE:
-
Phosphatidylethanolamine
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Acknowledgements
This work was supported by the National Natural Science Foundation of China (Grant No. 32160003), Doctor Support Project of Yan’an University (No. YDBK2019-44), Key Science and Technology Program at County Level of Shaanxi Province (2018XY-14), Graduate Education Innovation Plan of Yan’an University in 2021 (YCX2021083) and Yan’an Science and Technology Bureau Park and County Characteristic Economic Innovation Chain Project in 2019 (No. 2019ZDQY-035).
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National Natural Science Foundation of China, 32160003.
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ZD: designed the experiments; YJ and CZ: performed the experiments; TY, JL and JA: analyzed the data; ML: provided the material; XL and ZD: drafted and revised the manuscript. All authors have read and approved the final version of the manuscript.
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Zheng, Cc., Jiang, Yy., Yu, Tf. et al. Sphingopyxis yananensis sp. nov., a novel 2-nitropropane degrading bacterium isolated from a microbial fermentation bed substrate. Arch Microbiol 204, 529 (2022). https://doi.org/10.1007/s00203-022-03132-0
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DOI: https://doi.org/10.1007/s00203-022-03132-0