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Roseomonas rosulenta sp. nov., isolated from rice paddy soil

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Abstract

Three bacterial isolates, Gram-stain-negative, non-motile, coccobacilli-shaped bacteria, strains OP-27T, OP-5 and OP-30, were isolated from rice paddy soil. Phylogenetic analyses based on 16S rRNA gene sequences revealed that three isolates belonged to the genus Roseomonas, showing the highest sequence similarities to Roseomonas sediminicola FW-3T (98.1%) and Roseomonas lacus TH-G33T (98.0%). The genome size of strain OP-27T was 5.2 Mb in a single contig with DNA G+C content of 71.2%. The genome included 5164 predicted protein-coding genes, as well as 48 tRNA, 4 rRNA and 4 mRNA genes. The average nucleotide identity value between strain OP-27T and type strains of related species of the genus Roseomonas were 81.1–83.1%, and the digital DNA–DNA hybridization values of strain OP-27T and the related strains were 24.6–26.8%, respectively. The DNA–DNA hybridization values between strains OP-27T, OP-5 and OP-30 were 84–100% and its closest relative, Roseomonas sediminicola KACC 16616T was 21.1%. The major fatty acids were C18:1 ω7c, C18:1 2-OH and C16:0 and predominant quinone was Q-10. Based on its distinctive phenotypic, phylogenetic, and chemotaxonomic characteristics, the three strains are considered to represent novel species of the genus Roseomonas, for which the name Roseomonas rosulenta sp. nov. is proposed. The type strain is OP-27T (=KACC 21501T= NBRC 114497T).

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Data availability

The GenBank/EMBL/DDJB accession numbers for the 16S rRNA gene sequences of strains OP-27T, OP-5 and OP-30 are LC505025, LC505024 and LC505026. The GenBank/EMBL/DDBJ accession number for the draft genome sequence of strain OP-27T is JACADR000000000.

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Funding

This work was funded by the Strategic Initiative for Microbiomes in Agriculture and Food, Ministry of Agriculture, Food and Rural Affairs, Republic of Korea (as part of the Genome Technology to Business Translation Program). Grant number 918016-4.

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Authors and Affiliations

  1. Institute of Microbial Ecology and Resources, Mokwon University, 88, Doanbuk-ro, Seo-gu, Daejeon, Republic of Korea

    Hyo-Jin Lee & Kyung-Sook Whang

  2. Department of Microbial Biotechnology, Mokwon University, 88, Doanbuk-ro, Seo-gu, Daejeon, Republic of Korea

    Hyo-Jin Lee & Kyung-Sook Whang

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  1. Hyo-Jin Lee
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Contributions

HJL: performed experiments, data analysis and wrote the manuscript, HJL and KSW: designed research and revised the manuscript. All authors read and approved the final manuscript.

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Correspondence to Kyung-Sook Whang.

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Communicated by Erko Stackebrandt.

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Lee, HJ., Whang, KS. Roseomonas rosulenta sp. nov., isolated from rice paddy soil. Arch Microbiol 204, 445 (2022). https://doi.org/10.1007/s00203-022-03014-5

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