Abstract
A Gram-negative, aerobic, rod-shaped bacterium, designated strain CAU 1522T, was isolated from a sea sand sample collected from the Republic of Korea. Optimal growth of strain CAU 1522T ensued at 30 °C, pH 7.5, and 1.0% (w/v) NaCl. Strain CAU 1522T was affiliated to the genus Thalassococcus with high similarity to T. lentus KCTC 32084T (97.5%), T. profundi MCCC 1K03253T (96.5%), and T. halodurans JCM 13833T (96.1%) according to phylogenetic analysis based on 16S rRNA gene sequences. The whole genome of strain CAU 1522T was 3.7 Mb in length and included 7 contigs and 3599 protein-coding genes, with a G + C content of 65.4 mol%. The predominant cellular fatty acids were C18:1 ω6c and/or C18:1 ω7c (summed feature 8), with Q-10 being the sole isoprenoid quinone. The polar lipids included phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, phosphoglycolipid, and an unidentified lipid. These phenotypic, chemotaxonomic, and phylogenetic data support that strain CAU 1522T is a novel Thalassococcus species, for which the name T. arenae sp. nov. is proposed. The type strain is CAU 1522T (= KCTC 72545T = MCCC 1K04064T).
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Data availability
The 16S rRNA gene sequence and whole-genome sequence of strain CAU 1522T have been deposited in GenBank/EMBL/DDBJ under the accession numbers MN097943 and JAHRWL000000000, respectively.
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Acknowledgements
We thank Prof. Bernhard Schink and Prof. Aharon Oren for their recommendations for nomenclature proposed in this study.
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This work was supported by a grant from the National Institute of Biological Resources (NIBR), funded by the Ministry of Environment (MOE) of the Republic of Korea (NIBR201902203).
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Conceived and designed the experiments: WK; conducted the experiments: VW; analysed the data: J-HK, J-HY, and AS; contributed reagents, materials and analytical tools: WK, J-HY, and J-SL; and wrote the paper: VW and WK.
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Communicated by Erko Stackebrandt.
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Weerawongwiwat, V., Kim, JH., Lee, JS. et al. Thalassococcus arenae sp. nov. isolated from sea sand. Arch Microbiol 204, 409 (2022). https://doi.org/10.1007/s00203-022-03011-8
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DOI: https://doi.org/10.1007/s00203-022-03011-8