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Expression profile screening and bioinformatics analysis of CircRNA, LncRNA, and mRNA in HeLa cells infected with Chlamydia muridarum

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Abstract

We have previously shown that circRNAs in host cells are involved in the process of Chlamydia trachomatis infection. In this study we aimed to identify significantly altered circRNAs/lncRNAs/mRNAs in Chlamydia muridarum infected cells and investigate their biological functions in the interaction between Chlamydia muridarum and host cells. For this purpose, circRNA, lncRNA and mRNA expression profiles were screened and identified in HeLa cells with or without Chlamydia muridarum infection by microarray. Bioinformatics analyses including Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis and Gene Ontology (GO) analysis were then carried out and the circRNA-miRNA ceRNA network was constructed. The differentially expressed circRNAs and lncRNAs were selected for validation by RT-qPCR. The results shown that a total of 834 circRNAs, 2149 lncRNAs and 1283 mRNAs were found to be differentially expressed. Enrichment analysis of GO and KEGG showed that the dysregulated genes involved nuclear-transcribed mRNA catabolic process, protein binding, RNA catabolic process and translation, the MAPK signaling pathway, apoptosis, Toll-like receptor signaling pathway, cAMP signaling pathway and Notch signaling pathway may play important roles in Chlamydia infection. Our study provides a systematic outlook on the potential function of non-coding RNAs in the molecular basis of Chlamydia infection.

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Funding

This study was supported by the National Natural Science Foundation of China (Grant Nos. 31570178, 31100138) and the Natural Science Foundation of Tianjin (Grant No. 16JCQNJC11100). In addition, we thank the researchers in the Shanghai KangChen Biotech for their technical support.

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Authors and Affiliations

  1. Department of Dermatovenereology, Tianjin Medical University General Hospital, Tianjin, 300052, China

    Yuanjun Liu, Junqiu Xiang, Xinyue Hu & Huiping Wang

  2. NHC Key Laboratory of Hormones and Development, Tianjin Key Laboratory of Metabolic Diseases, Chu Hsien-I Memorial Hospital and Tianjin Institute of Endocrinology, Tianjin Medical University, Tianjin, 300134, China

    Yina Sun

Authors
  1. Yuanjun Liu
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  2. Junqiu Xiang
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  3. Xinyue Hu
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  4. Huiping Wang
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  5. Yina Sun
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Contributions

YL, HW and YS conceived and designed the experiments; YL, JX and XH performed the experiments; HW and YS analyzed the data; YL and YS wrote the paper.

Corresponding author

Correspondence to Yina Sun.

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The authors declare that they have no conflicts of interest.

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Communicated by Erko Stackebrandt.

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Liu, Y., Xiang, J., Hu, X. et al. Expression profile screening and bioinformatics analysis of CircRNA, LncRNA, and mRNA in HeLa cells infected with Chlamydia muridarum. Arch Microbiol 204, 352 (2022). https://doi.org/10.1007/s00203-022-02941-7

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  • DOI: https://doi.org/10.1007/s00203-022-02941-7

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