Abstract
Two extremely halophilic strains, designated SYSU A558-1T and SYSU A121-1, were isolated from a saline sediment sample collected from Aiding salt-lake, China. Cells of strains SYSU A558-1T and SYSU A121-1 were Gram-stain-negative, coccoid, and non-motile. The strains were aerobic and grew at NaCl concentration of 10–30% (optimum, 20–22%), at 20–55 °C (optimum, 37–42 °C) and at pH 6.5–8.5 (optimum, 7.0–8.0). Cells lysed in distilled water. The polar lipids were phosphatidyl choline, phosphatidylglycerol phosphate methyl ester, disulfated diglycosyl diether-1 and unidentified glycolipid. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that the two strains SYSU A558-1T and SYSU A121-1 were closely related to the membranes of the genus Haloterrigena. Phylogenetic and phylogenomic trees of strains SYSU A558-1T and SYSU A121-1 demonstrated a robust clade with Haloterrigena turkmenica, Haloterrigena salifodinae and Haloterrigena salina. The genomic DNA G + C content of strains SYSU A558-1T and SYSU A121-1 were 65.8 and 65.0%, respectively. Phenotypic, phylogenetic, chemotaxonomic and genome analysis suggested that the two strains SYSU A558-1T and SYSU A121-1 represent a novel species of the genus Haloterrigena, for which the name Haloterrigena gelatinilytica sp. nov. is proposed. The type strain is SYSU A558-1T (= KCTC 4259T = CGMCC 1.15953T).
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The draft genome sequences of isolates SYSU A558-1T and SYSU A121-1 are available at the NCBI genome database under the accessions JABUQZ000000000 and JABURA000000000, respectively. The three copies of 16S rRNA gene sequences of the isolate SYSU A558-1T are available under the accessions MT809061 (rrnA), MT809062 (rrnB) and MT809063 (rrnC), and that of isolate SYSU A121-1 under the accessions MT808217 (rrnA), MT808218 (rrnB) and MT808219 (rrnC). The rpoB' gene sequences of isolates SYSU A558-1T and SYSU A121-1 have been deposited in GenBank/EMBL/DDBJ under the accession numbers MT572483 and MT572484, respectively.
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Acknowledgements
The authors are grateful to Prof. Yu-Guang Zhou (CGMCC, China) for providing the reference type strains.
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This research was supported by National Natural Science Foundation of China (31800001, 31850410475), Science and Technology Development Special Fund Program of Guangdong Province (2017A030310206), Key Scientific Research Project of Colleges and Universities in Henan Province (20A180019), Henan Province University Youth Researcher Support Project (2021GGJS153), Key Technologies R&D Program of Henan Province (212102310232) and Key Technologies R&D Program of Nanyang city (KJGG079).
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S-XG and W-JL conceived the study; B-BL, NS, SC, Y-GX, Y-RZ and X-YY performed the experiments; B-BL, M-PNR, L-YW and NS wrote the draft manuscript; S-XG and W-JL finalized the manuscript.
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Liu, BB., Salam, N., Narsing Rao, M.P. et al. Haloterrigena gelatinilytica sp. nov., a new extremely halophilic archaeon isolated from salt-lake. Arch Microbiol 204, 176 (2022). https://doi.org/10.1007/s00203-022-02783-3
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DOI: https://doi.org/10.1007/s00203-022-02783-3