Abstract
Part of the native root nodule endophytic microflora referring to members of the genera Proteobacteria and Sphingobacteria were used to test their bioefficacy as seed biopriming. These were quantified for their plant growth promoting (PGP) attributes such as IAA production, P and K-solubilization and ACC deaminase production. Results showed that significantly highest IAA was produced by E. hormaechi RCT10. The highest P-solubilization was observed with S. maltophila RCT31 it was solubilizing all the substrate tri-calcium phosphate, di-calcium phosphate, and zinc phosphate. Significantly highest K-solubilization was observed with S. maltophila RCT31 followed by E. turicensis RCT5. However, the maximum zinc solubilization was reported with S. maltophila RCT31 followed by E. turicensis RCT5. The maximum ACC deaminase was quantified in the bacterium. Results revealed that the E. hormaechi RCT10 utilized seed leechates most effectively while root exudates were maximally utilized by S. maltophila RCT31. The pots experiment proves that S. maltophila RCT31 was the most effective bacterium and it was replication vis-à-vis field experiment. In particular, S. maltophila RCT31 holds strong potential to be possibly used as a bioformulation for the medicinal legume, as an economical and eco-friendly alternative to agrochemicals.
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References
Aeron A, Chauhan PS, Dubey RC, Maheshwari DK, Bajpai VK (2015) Root nodule bacteria from Clitoria ternatea L. are putative invasive nonrhizobial endophytes. Can J Microbiol 61(2):131–142
Aleksandrov VG, Blagodyr RN, Live IP (1967) Liberation of phosphoric acid from apatite by silicate bacteria. Microbiol 29:111–114
Alexander G, Greer SE, Zhuline IB (2000) Energy taxis is a dominant behavior in Azospirillum brasilense. J Bacteriol 182:6042–6048
Bhatia S, Maheshwari DK, Dubey RC, Arora DS, Bajpai VK, Kang SC (2008) Beneficial effect of fluorescent Pseudomonads on seed germination, growth promotion and suppression of charcoal rot in ground nut (Arachis hypogea L.). J Microbiol Biotechnol 18(9):1578–1583
Bric JM, Bostock RM, Siliverstone SE (1991) Rapid in situ assay for indoleacetic acid production by bacteria immobilized on a nitrocellulose membrane. Appl Environ Microbiol 57(2):535–538
Clancy M, Madill KA, Wood JM (1981) Genetic and biochemical requirement for chemotaxis to L. praline in Escherichia coli. J Bacteriol 146:902–906
Dey R, Pal KK, Bhatt DM, Chauhan SM (2004) Growth promotion and yield enhancement of peanut (Arachis hypogaea L.) by application of plant growth-promoting rhizobacteria. Microbiol Res 159:371–394
Glick BR (2012) Plant growth-promoting bacteria: mechanisms and applications. Scientifica. https://doi.org/10.6064/2012/963401
Honma M, Shimomura T (1978) Metabolism of 1-aminocyclopropane-1-carboxylic acid. Agric Biol Chem 43:1825–1831
Muresu R, Polone E, Sulas L, Baldan B, Tondello A, Delogu G, Cappuccinelli P, Alberghini S, Benhizia Y, Benhizia H, Benguedouar A, Mori B, Calamassi R, Dazzo FB, Squartini A (2008) Coexistence of predominantly non-culturable rhizobia with diverse endophytic bacterial taxa within nodules of wild legumes. FEMS Microbiol Ecol 63:383–400
Muthukumar A, Udhayakumar R, Naveenkumar R (2017) Role of endophytes in plant disease control. In: Maheshwari DK, Annapurna K (eds) Endophytes: crop productivity and protection. Sustainable development and biodiversity. Springer, Berlin
Palaniappan P, Chauhan PS, Saravanan VS, Anandham R, Sa TM (2010) Isolation and characterization of plant growth promoting endophytic bacterial isolates from root nodule of Lespedeza sp. Biol Fertil Soil 46:807–816. https://doi.org/10.1007/s00374-010-0485-5
Reinhold-Hurek B, Hurek T (2011) Living inside plants: bacterial endophytes. Curr Opin Plant Biol 14:435–443
Schulz B, Boyle C (2006) What are endophytes? Microbial root endophytes. Springer, Berlin, pp 1–13
Sharma SK, Sharma MP, Ramesh A, Joshi OP (2012) Characterization of zinc solubilizing Bacillus isolates and their potential to influence zinc assimilation in soybean seeds. J Microbiol Biotechnol 22:352–359. https://doi.org/10.4014/jmb.1106.05063
Weller DM (1988) Biological-control of soilborne plant-pathogens in the rhizosphere with bacteria. Annu Rev Phytopathol 26:379–407
Acknowledgement
DKM and AA wishes to acknowledge funds received for his laboratory from Uttarakhand Council for Science and Technology (CS&T/R&D/LS-19/06 UCOST, DST, Govt. of Uttarakhand, Dehradun), from University Grants Commission (33-201/2007(SR), UGC, New Delhi). AA and DKM wrote the first draft of the manuscript and VSM designed figures, tabulation and finalizing the manuscript. We thank Prof. Erko Stackebrandt, Editor-in-Chief, Archives of Microbiology for his valuable comments and suggestions.
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Aeron, A., Maheshwari, D.K. & Meena, V.S. Endophytic bacteria promote growth of the medicinal legume Clitoria ternatea L. by chemotactic activity. Arch Microbiol 202, 1049–1058 (2020). https://doi.org/10.1007/s00203-020-01815-0
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DOI: https://doi.org/10.1007/s00203-020-01815-0