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Pseudomonas qingdaonensis sp. nov., an aflatoxin-degrading bacterium, isolated from peanut rhizospheric soil

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Abstract

A Gram-stain-negative, aerobic, mobile, and rod-shaped bacterium, designated JJ3T, was isolated from peanut rhizospheric soil in Qingdao, Shandong Province, China, and was characterized using a polyphasic approach. Strain JJ3T grew at 4–40 °C, at pH 5.0–9.0 and 0–4% NaCl. The strain was positive for both catalase and oxidase tests, and was able to degrade aflatoxin B1. According to the 16S rRNA gene sequence comparisons, the strain JJ3T was identified as a member of the genus Pseudomonas and was most closely related to Pseudomonas japonica JCM 21532T and Pseudomonas alkylphenolica JCM 16553T with sequence similarity of 99.0% and 98.9%, respectively. A multilocus sequence analysis (MLSA) of concatenating 16S rRNA, gyrB and rpoD gene sequences showed that strain JJ3T belonged to the Pseudomonas putida subcluster. Genomic comparison of strain JJ3T with its closest phylogenetic type strain using average nucleotide index (ANI) and digital DNA–DNA relatedness revealed 76.7–82.9% and 20.2–37.1%, respectively. All values were distinctly lower than the thresholds established for species differentiation. The predominant cellular fatty acids of strain JJ3T were C17:0 cyclo (24.0%), C16:0 (21.4%), summed features 3 (C16:1ω7c and/or C16:1ω6c) (11.5%) and summed features 8 (C18:1ω7c and/or C18:1ω6c) (10.5%). The major polar lipids of strain JJ3T were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The physiological, biochemical, and genetic characteristics support the assignment of JJ3T to the genus Pseudomonas, but are different to those of phylogenetically neighboring species to represent a novel species. The name Pseudomonas qingdaonensis sp. nov. is proposed, with JJ3T (= JCM 32579T = KCTC 62384T = CGMCC 1.16493T) as the type strain.

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Acknowledgements

This work was supported by the grants from the Natural Science Foundation of Shandong Province (ZR2016CM43, ZR2016YL021, ZR2017MC060, ZR2017MC062), the Innovation Project of AgriculturalScience and Technology of Shandong Academy of Agricultural Sciences (CXGC2018E21), the Major Scientific and Technological Achievements Cultivation Program of Shandong Academy of Agricultural Sciences (2016CGPY10), Shandong Major Agricultural Application Technology Innovation Project.

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Authors and Affiliations

  1. Shandong Peanut Research Institute, Wannianquan Road 126, Qingdao, 266100, China

    Ming-Qing Wang, Zhen Wang, Li-Na Yu, Chu-Shu Zhang, Jie Bi & Jie Sun

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  1. Ming-Qing Wang
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  2. Zhen Wang
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  3. Li-Na Yu
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  4. Chu-Shu Zhang
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Correspondence to Ming-Qing Wang or Jie Sun.

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Communicated by Erko Stackebrandt.

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The accession numbers of the 16S rRNA, gyrB, and rpoD genes for the type strain JJ3T in GenBank are MG589917, MH758785, and MH758786, respectively. The genome has been deposited in GenBank under the accession number of PHTD00000000 for strain JJ3T. The digital protologue database (DPD) Taxon Number of strain JJ3T is TA00689.

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Wang, MQ., Wang, Z., Yu, LN. et al. Pseudomonas qingdaonensis sp. nov., an aflatoxin-degrading bacterium, isolated from peanut rhizospheric soil. Arch Microbiol 201, 673–678 (2019). https://doi.org/10.1007/s00203-019-01636-w

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