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Pseudomonas benzenivorans sp. nov. and Pseudomonas saponiphila sp. nov., Represented by Xenobiotics Degrading Type Strains

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Abstract

Two strains of gram-negative bacteria isolated because of their abilities to decompose xenobiotic compounds were subjected to a polyphasic taxonomic study. On the basis of 16S rRNA gene sequence analysis, the two strains were found to belong to the genus Pseudomonas. Benzene degrading strain DSM 8628T was moderately related to P. flavescens NCPP 3063T (98.3% similarity), P. monteilii CIP 104883T, and P. plecoglossicida FPC 951T (98.1%). Strain DSM 9751T capable to grow with cetyltrimethylammonium chloride as the sole carbon source showed the highest similarity values with P. tremae CFBP 2341T and P. meliae MAFF 301463T (98.0%), both related to Pseudomonas syringae. The fatty acid pattern of strain DSM 8628T was distinct from patterns of other members of the genus Pseudomonas in combining a high ratio of 3OH-C12:1 (5.1%), a low ratio of 2OH-C12:0 (0.2%) and a relatively low ratio of C18:1ω7c (23.8%). On the basis of phylogenetic analysis, physiological properties and the composition of whole cell fatty acids, two novel species, Pseudomonas benzenivorans sp. nov. with the type strain DSM 8628T (=CIP 109857T) and Pseudomonas saponiphila sp. nov. with the type strain DSM 9751T (=CIP 109856T), are proposed.

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Acknowledgements

We are thankful to E. Stackebrandt for supporting the phylogenetic analyses, to J. Spain and W.F. Guerin for donating the strains to the DSMZ for deposition in the open collection. Performance of fatty acid analyses by R.M. Kroppenstedt, help during plasmid isolation by I. Schröder and excellent technical assistance by M. Kopitz and P. Aumann are acknowledged.

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Authors and Affiliations

  1. DSMZ—Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Inhoffenstraße 7b, 30124, Braunschweig, Germany

    Elke Lang, Melanie Burghartz, Stefan Spring, Jolanthe Swiderski & Cathrin Spröer

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  1. Elke Lang
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  2. Melanie Burghartz
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  3. Stefan Spring
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  4. Jolanthe Swiderski
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  5. Cathrin Spröer
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Correspondence to Elke Lang.

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Lang, E., Burghartz, M., Spring, S. et al. Pseudomonas benzenivorans sp. nov. and Pseudomonas saponiphila sp. nov., Represented by Xenobiotics Degrading Type Strains. Curr Microbiol 60, 85–91 (2010). https://doi.org/10.1007/s00284-009-9507-7

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  • DOI: https://doi.org/10.1007/s00284-009-9507-7

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