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Novosphingobium endophyticum sp. nov. isolated from roots of Glycyrrhiza uralensis

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Abstract

A novel Gram-staining-negative, non-motile, rod-shaped and aerobic bacterial strain, designated EGI 60015T, was isolated from healthy roots of Glycyrrhiza uralensis F. collected from Yili County, Xinjiang Province, Northwest China. The 16S rRNA gene sequence of strain EGI 60015T was found to show 97.6 % sequence similarity with Novosphingobium pentaromativorans US6-1T. The phylogenetic analysis based on 16S rRNA gene sequences showed that the strain formed a clade with N. pentaromativorans US6-1T in the neighbor-joining tree. Q-10 was identified as the respiratory quinone of strain EGI 60015T. The major fatty acids were summed feature 8 (C18:1 ω6c and/or C18:1 ω7c; 55.04 %), summed feature 4 (C17:1 anteiso B and/or iso I; 18.34 %) and summed feature 3 (C16:1 ω6c and/or C16:1 ω7c; 8.53 %). The polar lipids detected were phosphatidylethanolamine, phosphatidylmethylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine and sphingoglycolipids. The DNA G+C content of strain EGI 60015T was determined to be 66.6 mol%. The genomic DNA relatedness value between EGI 60015T and N. pentaromativorans US6-1T (54 %) was below the 70 % limit for species identification. Based on the result of the molecular studies supported by its morphological, physiological, chemotaxonomic and other differentiating phenotypic characteristics, strain EGI 60015T was considered to represent a novel species within the genus Novosphingobium, for which the name Novosphingobium endophyticum sp. nov. is proposed. The type strain is EGI 60015T (=CGMCC 1.15095T = KCTC 42486T = DSM 29948T).

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Acknowledgments

We are grateful to Prof. Dr. Takuji Kudo (JCM) for providing the reference type strain. This research was supported by the National Natural Science Foundation of China (Nos. 81102806, 31200008 and 31400009), projects of China tobacco Yunnan Industrial Co. Ltd. (Nos. 2012JC07, 2012FL02 and 2014YL01), the Hundred Talents Program of Chinese Academy of Sciences, the High-level Talents Program of Xinjiang Autonomous Region and the West Light Foundation of Chinese Academy of Sciences. W.-J. Li was also supported by Guangdong Province Higher Vocational Colleges & Schools Pearl River Scholar Funded Scheme (2014).

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    Authors and Affiliations

    1. Kunming Medical University Haiyuan College, Kunming, 650106, People’s Republic of China

      Yan-Qiong Li

    2. Key Laboratory of Biogeography and Bioresource in Arid Land, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Ürümqi, 830011, People’s Republic of China

      Li Li, Jian-Wei Guo & Wen-Jun Li

    3. China Tobacco Yunnan Industrial Co. Ltd., Kunming, 650231, People’s Republic of China

      Wei Chen, Yan-Qing Duan & Rui Gao

    4. Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, People’s Republic of China

      Yan-Qiong Li, Salam Nimaichand & Wen-Jun Li

    5. State Key Laboratory of Biocontrol and Guangdong Key Laboratory of Plant Resources, College of Ecology and Evolution, Sun Yat-Sen University, Guangzhou, 510275, People’s Republic of China

      Salam Nimaichand & Wen-Jun Li

    6. Key Laboratory of Higher Quality and Efficient Cultivation and Security Control of Crops for Yunnan Province, Honghe University, Mengzi, 661100, People’s Republic of China

      Jian-Wei Guo

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    Communicated by Erko Stackebrandt.

    Yan-Qiong Li, Li Li and Wei Chen have contributed equally to this work.

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    Li, YQ., Li, L., Chen, W. et al. Novosphingobium endophyticum sp. nov. isolated from roots of Glycyrrhiza uralensis . Arch Microbiol 197, 911–918 (2015). https://doi.org/10.1007/s00203-015-1124-1

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