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Diversity and bioprospecting of culturable actinomycetes from marine sediment of the Yellow Sea, China

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Abstract

Marine actinomycetes are a potential source of a wide variety of bioactive natural products. In this work, seven pretreatments, three selective isolation media, and five artificial seawater concentrations were used to isolate actinomycetes from the sediments collected from Yellow Sea, China. Statistical analysis showed that only the isolation medium strongly affected the total and bioactive numbers of actinomycete isolates. A total of 613 actinobacterial strains were isolated and screened for antimicrobial activities; 154 isolates showed activity against at least one of nine test drug-resistant microorganisms. Eighty-nine representatives with strong antimicrobial activity were identified phylogenetically based on 16S rRNA gene sequencing, which were assigned to five different actinomycete genera Streptomyces, Kocuria, Saccharomonospora, Micromonospora, and Nocardiopsis. Using PCR-based screening for six biosynthetic genes of secondary metabolites, all 45 isolates with acute activity have at least one biosynthetic gene, 28.8 % of which possess more than three biosynthetic genes. As a case, strain SMA-1 was selected for antimicrobial natural product discovery. Three diketopiperazine dimers including a new compound iso-naseseazine B (1) and two known compounds naseseazine B (2) and aspergilazine A (3) were isolated by bioassay-guided separation. These results suggested that actinomycetes from marine sediments are a potential resource of novel secondary metabolites and drugs.

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Acknowledgments

This work was supported by the National High Technology Research and Development Program (“863” Program: 2012AA02A701), National Basic Research Program of China (973 Program, Grant no. 2012CB721104), the National Natural Science Foundation of China (Grant Nos. 31170101, 31100073, 31270148, and 21305150), and Shanghai Natural Science Foundation (Grant No. 12ZR1435700).

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Authors and Affiliations

  1. Key Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200032, China

    Zhi-Qiang Xiong, Qiao-Xia Liu, Zhao-Long Pan, Na Zhao, Zhi-Xiang Feng & Yong Wang

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  1. Zhi-Qiang Xiong
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  2. Qiao-Xia Liu
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  3. Zhao-Long Pan
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  4. Na Zhao
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  5. Zhi-Xiang Feng
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  6. Yong Wang
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Correspondence to Yong Wang.

Additional information

Communicated by Erko Stackebrandt.

Zhi-Qiang Xiong and Qiao-Xia Liu have contributed equally to this work.

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Xiong, ZQ., Liu, QX., Pan, ZL. et al. Diversity and bioprospecting of culturable actinomycetes from marine sediment of the Yellow Sea, China. Arch Microbiol 197, 299–309 (2015). https://doi.org/10.1007/s00203-014-1059-y

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  • DOI: https://doi.org/10.1007/s00203-014-1059-y

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