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Morpho-physiological and genetic characteristics of a salt-tolerant mutant line in soybean (Glycine max L.)

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Abstract

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One major quantitative trait loci and candidate gene for salt tolerance were identified on chromosome 3 from a new soybean mutant derived from gamma-ray irradiation, which will provide a new genetic resource for improving soybean salt tolerance.

Abstract

Soil salinity is a worldwide problem that reduces crop yields, but the development of salt-tolerant crops can help overcome this challenge. This study was conducted with the purpose of evaluating the morpho-physiological and genetic characteristics of a new salt-tolerant mutant KA-1285 developed using gamma-ray irradiation in soybean (Glycine max L.). The morphological and physiological responses of KA-1285 were compared with salt-sensitive and salt-tolerant genotypes after treatment with 150 mM NaCl for two weeks. In addition, a major salt tolerance quantitative trait locus (QTL) was identified on chromosome 3 in this study using the Daepung X KA-1285 169 F2:3 population, and a specific deletion was identified in Glyma03g171600 (Wm82.a2.v1) near the QTL region based on re-sequencing analysis. A kompetitive allele-specific PCR (KASP) marker was developed based on the deletion of Glyma03g171600 which distinguished the wild-type and mutant alleles. Through the analysis of gene expression patterns, it was confirmed that Glyma03g171700 (Wm82.a2.v1) is a major gene that controls salt tolerance functions in Glyma03g32900 (Wm82.a1.v1). These results suggest that the gamma-ray-induced mutant KA-1285 has the potential to be employed for the development of a salt-tolerant cultivar and provide useful information for genetic research related to salt tolerance in soybeans.

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Data availability

The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.

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Funding

This work was carried out with the support of the Cooperative Research Program for Agriculture Science and Technology Development (Project No. PJ015013), the Rural Development Administration, Republic of Korea.

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Author notes

  1. Chang Yeok Moon and Byeong Hee Kang have contributed equally to this work.

Authors and Affiliations

  1. Department of Applied Plant Science, Chonnnam National University, Gwangju, 61186, Republic of Korea

    Chang Yeok Moon, Byeong Hee Kang, Woon Ji Kim, Sreeparna Chowdhury, Sehee Kang, Han-Yong Kim & Bo-Keun Ha

  2. BK21 Interdisciplinary Program in IT-Bio Convergence System, Chonnam National University, Gwangju, 61186, Republic of Korea

    Chang Yeok Moon, Byeong Hee Kang, Sehee Kang & Bo-Keun Ha

  3. Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup, 56212, Republic of Korea

    Woon Ji Kim & Soon-Jae Kwon

  4. Department of Applied Biosciences, Kyungpook National University, Daegu, 41566, Republic of Korea

    Jeong-Dong Lee

  5. National Institute of Crop Science, RDA, Wanju, 55365, Republic of Korea

    Hyeon-Seok Lee

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  1. Chang Yeok Moon
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Contributions

Writing—original draft preparation, CYM; writing—review and editing, BHK; methodology, WKK, SC and SK; resources, JDL and SJK; supervision, H-SL and B-KH; funding acquisition, H-YK, H-SL and B-KH All authors have read and agreed to the published version of the manuscript.

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Correspondence to Hyeon-Seok Lee or Bo-Keun Ha.

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Communicated by Istvan Rajcan.

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Moon, C.Y., Kang, B.H., Kim, W.J. et al. Morpho-physiological and genetic characteristics of a salt-tolerant mutant line in soybean (Glycine max L.). Theor Appl Genet 136, 166 (2023). https://doi.org/10.1007/s00122-023-04408-9

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