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Fine mapping of reduced height locus RHT26 in common wheat

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Abstract

Key message

We fine mapped RHT26 for plant height in wheat, confirmed its genetic effects in a panel of wheat cultivars and predicted candidate genes.

Abstract

Development of wheat cultivars with appropriate plant height (PH) is an important goal in breeding. Utilization of semi-dwarfing genes Rht-B1b and Rht-D1b triggered wheat Green Resolution in the 1960s. Since these genes also bring unfavorable features, such as reduced coleoptile length and grain weight, it is necessary to identify alternative reduced height genes without yield penalty. Here we constructed a high-density genetic map of a recombinant inbred line population derived from the cross of Zhongmai175 and Lunxuan987 and detected a stable genetic locus for PH, designated RHT26, on chromosome arm 3DL in all of six environments, accounting for 6.8–14.0% of the phenotypic variances. RHT26 was delimited to an approximate 1.4 Mb physical interval (517.1–518.5 Mb) using secondary mapping populations derived from 22 heterozygous recombinant plants and 24 kompetitive allele-specific PCR markers. Eleven high-confidence genes were annotated in the physical interval according to the Chinese Spring reference genome, and four of them were predicted as candidates for RHT26 based on genome and transcriptome sequencing analyses. We also confirmed that RHT26 had significant effects on PH, but not grain yield in a panel of wheat cultivars; its dwarfing allele has been frequently used in wheat breeding. These findings lay a sound foundation for map-based cloning of RHT26 and provide a breeding-applicable tool for marker-assisted selection.

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Data availability

The datasets generated during the current study are available from the corresponding author Shuanghe Cao (caoshuanghe@caas.cn) on reasonable request.

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Acknowledgements

The authors are grateful to Prof Robert McIntosh, Plant Breeding Institute, University of Sydney, for revising this manuscript. This work was supported by National Key Research and Development Program of China (2022YFF1002901), the National Natural Science Foundation of China (91935304 and 32272182) and Science and Technology Innovation Program of Chinese Academy of Agricultural Sciences.

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Authors and Affiliations

  1. Institute of Crop Sciences, National Wheat Improvement Center, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, Beijing, 100081, China

    Jie Song, Lei Li, Bingyan Liu, Yachao Dong, Yan Dong, Siyang Liu, Xumei Luo, Mengjing Sun, Zhongqiu Ni, Shuaipeng Fei, Xianchun Xia & Shuanghe Cao

  2. Crop Research Institute, Shandong Academy of Agricultural Sciences, 202 Gongye North Road, Jinan, 250100, Shandong, China

    Faji Li

  3. State Key Laboratory of Agrobiotechnology, Key Laboratory of Crop Heterosis and Utilization, Beijing Key Laboratory of Crop Genetic Improvement, College of Agronomy, China Agricultural University, 2 Yuanmingyuan West Road, Beijing, 100094, China

    Zhongfu Ni

  4. International Maize and Wheat Improvement Center (CIMMYT) China Office, c/o, CAAS, 12 Zhongguancun South Street, Beijing, 100081, China

    Zhonghu He

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  1. Jie Song
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  2. Lei Li
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Contributions

JSong and SHC wrote the draft manuscript; JS performed the experiments; LL, BYL, YCD, YD, SYL, XML, MJS, ZQN and SPF participated in field trials; JS, SHC and FJL analyzed the data; and SHC, XCX, ZFN and ZHH designed the project.

Corresponding authors

Correspondence to Zhonghu He or Shuanghe Cao.

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We declare no conflicts of interest in regard to this manuscript.

Ethical standards

These experiments complied with the ethical standards in China.

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Communicated by Peter Langridge.

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Song, J., Li, L., Liu, B. et al. Fine mapping of reduced height locus RHT26 in common wheat. Theor Appl Genet 136, 62 (2023). https://doi.org/10.1007/s00122-023-04331-z

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