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A novel mutation in TFL1 homolog sustaining determinate growth in cucumber (Cucumis sativus L.)

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Abstract

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BSA-seq combined with whole-genome resequencing map-based cloning delimited the cucumber det-novel locus into a 44.5 kb region in chromosome 6 harboring a putative candidate gene encoding a phosphatidylethanolamine-binding protein (CsCEN).

Abstract

Determinate and indeterminate growth habits of cucumber can affect plant architecture and crop yield. The TERMINAL FLOWER 1 (TFL1) controls determinate/indeterminate growth in Arabidopsis. In this study, a novel mutation in cucumber TFL1 homolog (CsCEN) has shown to regulate determinate growth and product of terminal flowers in cucumber (Cucumis sativus L.), which is similar to the function of CsTFL1 as previously reported. Genetic analysis in two determinate genotypes (D226 and D082) and indeterminate genotype (CCMC) revealed that a single recessive gene is responsible for this determinate growth trait. With the combination of BSA-seq and whole-genome resequencing, the locus of determinate-novel (det-novel) trait was mapped to a 44.5 kb genomic region in chromosome 6. Sequence alignment identified one non-synonymous SNP mutation (A to T) in the third exon of CsCEN, resulting in an amino acid substitution (Thr to Pro), suggesting that determinate growth might be controlled by a novel gene CsCEN (Csa6G152360) which differed from the reported CsTFL1 gene. The CsCEN expression level in shoot apexes and axillary buds was significantly lower in D226 compared to CCMC, suggesting its essential role in sustaining indeterminate growth habit. Identification and characterization of the CsCEN in the present study provide a new insight into plant architecture modification and development of cucumber cultivars suited to mechanized production system.

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Acknowledgements

A Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions and the Belt—Road project by Jiangsu Provincial Government; Also partly funded by National Key Research and Development Program of China (2018YFD1000804), Jiangsu Agricultural Innovation of New Cultivars ‘Innovation and application of cucumber cultivars with high quality and multi disease resistances (No. PZCZ201719),’ National Natural Science Foundation of China (No. 31672168) and Natural Science Foundation of Jiangsu Province (BK20191312).

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Author notes

  1. Martin Kagiki Njogu, Fan Yang and Ji Li have contributed equally to this work.

Authors and Affiliations

  1. State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China

    Martin Kagiki Njogu, Fan Yang, Ji Li, Xueyan Wang & Jinfeng Chen

  2. Department of Plant Science, Chuka University, P.O. Box 109-60400, Chuka, Kenya

    Martin Kagiki Njogu

  3. Department of Crops Horticulture and Soil Science, Egerton University, Njoro, Kenya

    Joshua Otieno Ogweno

Authors
  1. Martin Kagiki Njogu
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  2. Fan Yang
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  3. Ji Li
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  4. Xueyan Wang
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  5. Joshua Otieno Ogweno
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  6. Jinfeng Chen
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Contributions

JFC and JOO conceived and supervised the projects. MKN, FY and XYW collected phenotypic data from field. MKN and FY performed the experiments. MKN, FY and JL analyzed the data and wrote manuscript. JL, JFC and JOO contributed to revising the manuscript. All authors reviewed and contributed in drafting the manuscript. All authors read and approved the final manuscript.

Corresponding authors

Correspondence to Joshua Otieno Ogweno or Jinfeng Chen.

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The experiments were performed in accordance with all relevant Chinese laws.

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Njogu, M.K., Yang, F., Li, J. et al. A novel mutation in TFL1 homolog sustaining determinate growth in cucumber (Cucumis sativus L.). Theor Appl Genet 133, 3323–3332 (2020). https://doi.org/10.1007/s00122-020-03671-4

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  • DOI: https://doi.org/10.1007/s00122-020-03671-4

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