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Phenotypic, genetic, and molecular function of msc-2, a genic male sterile mutant in pepper (Capsicum annuum L.)

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Abstract

Key message

Bulked segregant analysis and fine mapping delimited the pepper genic male sterile (msc-2) locus into a 336 kb region on chromosome 5. A strong candidate gene, Capana05g000766, a homolog of AtMS1, was indentified in this region.

Abstract

Genic male sterility (msc-2) is used to produce hybrid seeds in Northern China. However, no co-segregated markers have been reported or candidate genes controlling this trait have been cloned. Here, bulked segregant analysis and genotyping of an F2 population and a 18Q5431AB line were employed to fine map msc-2, which was delimited to a 336 kb region. In this region, Capana05g000766 was a homolog of AtMS1, which encodes a plant homeodomain finger involved in tapetum development. A “T” deletion in the Capana05g000766 locus leads to a premature stop codon, which may cause a loss-of-function mutation. Real-time PCR analysis revealed that Capana05g000766 was an anther-specific gene and down-regulation of the gene resulted in male sterility. Therefore, Capana05g000766 was identified as the strongest candidate gene for the msc-2 locus. Allelism tests showed that msc-1 and msc-2 were nonallelic, and bimolecular fluorescence complementation analysis indicated that the two genes did not interact directly with each other at the protein level. As msc-1 and msc-2 are homologs of AtDYT1 and AtMS1 in Arabidopsis, they may play similar roles in tapetum development in genic male sterile peppers, and Msc-1 might be up stream of Msc-2 in the regulation of other genes involved in tapetum development.

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Abbreviations

AMS:

ABORTED MICROSPORES

bHLH:

Basic helix-loop-helix

BiFC:

Bimolecular fluorescence complementation

BSA:

Bulked segregant analysis

Chr.:

Chromosome

CAPs:

Cleaved amplified polymorphic sequence

CMS:

Cytoplasmic male sterility

DYT1:

DYSFUNCTIONAL TAPETUM1

GMS:

Genic male sterility

HRM:

High-resolution melting

LZ:

Leucine zipper

MS1:

MALE STERILE1

MS188:

MALE STERILITY188

PHD:

Plant homeodomain

PCD:

Programmed cell death

qRT:

Quantitative reverse transcription

SNP:

Single nucleotide polymorphism

SLAF-Seq:

Specific locus amplified fragment sequencing

SSR:

Simple sequence repeat

TDF1:

DEFECTIVE IN TAPETAL DEVELOPMENT AND FUNCTION1

VIGS:

Virus-induced gene silencing

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Acknowledgements

This work is supported by National Natural Science Foundation of China (31872097), Modern Agricultural Industry Technology System of Beijing Fruit Vegetables Innovation Team (BAIC01-2019) and The Construction of Beijing Science and Technology Innovation and Service Capacity in Top Subjects (CEFF-PXM2019_014207_000032).

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  1. Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, Department of Vegetable Science, College of Horticulture, China Agricultural University, Beijing, 100193, China

    Qing Cheng, Ting Li, Yixin Ai, Qiaohua Lu, Yihao Wang, Lang Wu, Jinqiu Liu, Liang Sun & Huolin Shen

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  1. Qing Cheng
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  2. Ting Li
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Contributions

QC performed most of the experiments, analyzed the data and drafted the manuscript. TL performed the semi-thin paraffin section analysis. YA and QL performed the qRT-PCR analysis. YW, LW, and LJ performed VIGS experiments. LS modified the manuscript. HS designed and directed the entire study. All authors have read and approved the final manuscript.

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Correspondence to Huolin Shen.

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Cheng, Q., Li, T., Ai, Y. et al. Phenotypic, genetic, and molecular function of msc-2, a genic male sterile mutant in pepper (Capsicum annuum L.). Theor Appl Genet 133, 843–855 (2020). https://doi.org/10.1007/s00122-019-03510-1

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